目的 探讨新疆哈萨克族食管癌中Survivin基因表达及其与半胱氨酸蛋白Caspase-9、Caspase-3基因的关系。方法 首先从52例哈萨克族食管癌标本中筛选出34例Survivin mRNA差异表达的标本（癌组织中的表达量高于远端无癌组织），采用RT-PCR、Western blotting方法检测半胱氨酸蛋白Caspase-9、Caspase-3 mRNA和蛋白的表达。结果 1. Survivin在新疆哈萨克族食管癌组织高表达，差异具有统计学意义（P <0.05）；2. 在Survivin差异表达的34例标本中, 61.8%的肿瘤组织半胱氨酸蛋白Caspase-9低表达，并且 Survivin mRNA与Caspase-9 mRNA的表达呈负相关（r = -0.244，P <0.05）；3. 哈萨克族食管癌组织中，Caspase-9的蛋白活化降低（P <0.05）。结论 Survivin表达上调在新疆哈萨克族食管癌的发生、发展过程中发挥重要作用，Survivin可能通过直接抑制Caspase-9的转录以及抑制其活化水平发挥调控细胞凋亡的作用。

Objective To investigate the high expressed Survivin and its relationship with the apoptosis proteins in esophageal cancer of Kazakh in Xinjiang. Methods Thirty-four Survivin mRNA differential expression specimens from 52 cases of Kazakh’s esophageal cancer specimen (cancerous tissue expression in quantity than distal normal tissue) were screened. Caspase-9 and Caspase-3 gene and protein expression were detected by RT-PCR and Westernblotting respectively. Results 1. Survivin overexpressed in Kazakh’s esophageal cancer tissue, compared with distal normal tissue, differences were statistically significant (P <0.05); 2. 61.8% of the specimens had Caspase-9 low expression in 34 case specimens with Survivin mRNA differential expression, and the expression of Survivin was negatively correlated to the Caspase-9 expression（r = -0.244，P <0.05）; 3. Caspase-9 protein activation significantly decreased（P <0.05）. Conclusion The overexpression of Survivin may play an important role in the origin and development of Xinjiang Kazakh’sesophageal cancer. The function of Survivin in regulation of apoptosis may be related to direct inhibition of Caspase-9 and activation of transcription.