脑红蛋白在成年牦牛不同组织细胞中的分布

曹亮 刘霞 石宁宁 李盛杰 杜晓华*

doi:10.3969/j.issn.0529-1356.2013.01.01.013

解剖学报 ›› 2013, Vol. 44 ›› Issue (1) : 68-72. DOI: 10.3969/j.issn.0529-1356.2013.01.01.013

细胞和分子生物学

脑红蛋白在成年牦牛不同组织细胞中的分布

曹亮¹刘霞²石宁宁²李盛杰²杜晓华^1*

作者信息 +

Distribution of neuroglobin in different tissues and cells of the adult yak

CAO Liang¹LIU Xia²SHI Ning-ning²LI Sheng-jie²DU Xiao-hua¹*

Author information +

文章历史 +

摘要

目的探讨脑红蛋白（NGB）在成年牦牛不同组织细胞中的分布特征。方法选择健康成年牦牛8只，利用免疫组织化学染色SP法，观察NGB在成年牦牛不同组织细胞中的分布特征。结果在成年牦牛体内，NGB主要表达于神经细胞，肾上腺、腺垂体及胰岛组织细胞，雄性生殖系统睾丸、睾丸输出小管及附睾管上皮细胞。NGB免疫阳性物质定位于细胞质。本研究在哺乳动物消化系统皱胃胃底腺分泌细胞及十二指肠腺分泌细胞中发现有NGB阳性表达。结论在牦牛神经系统、内分泌系统、生殖系统以及消化系统组织细胞中有高表达，提示NGB在这些组织的氧利用及功能活动中发挥作用。

Abstract

Objective To explore the localization of neuroglobin (NGB) in adult yak tissues and cells. Methods Eight health adult yaks were used in this study. NGB expression in adult yak tissues and cells was localized using the immunohistochemical SP technique. Results NGB-positive cells were mainly distributed in nerve cells, adrenal gland, adenohypophysis and pancreas islet, testis, efferent ductules of testis and epididymal epithelium of genital system. NGB-immunoreactive product was located in the cytoplasm. The NGB-positive cells were found in the fundic glands of abomasum and duodenal glands.Conclusion The high expression of NGB in tissues and cells of nervous system, endocrine system, genital system and alimentary system in yak suggested that NGB might play an important role in the utilization of oxygen and physiological functions.

导出引用

曹亮刘霞石宁宁李盛杰杜晓华*. 脑红蛋白在成年牦牛不同组织细胞中的分布[J]. 解剖学报. 2013, 44(1): 68-72 https://doi.org/10.3969/j.issn.0529-1356.2013.01.01.013

CAO Liang LIU Xia SHI Ning-ning LI Sheng-jie DU Xiao-hua*. Distribution of neuroglobin in different tissues and cells of the adult yak[J]. Acta Anatomica Sinica. 2013, 44(1): 68-72 https://doi.org/10.3969/j.issn.0529-1356.2013.01.01.013

参考文献

［1］ Burmester T, Weich B, Reinhardt S, et al. A vertebrate globin expressed in the brain ［J］. Nature, 2000, 407(6803): 520-523.

［2］Reuss S, Saaler-Reinhardt S, Weich B, et al. Expression analysis of neuroglobin mRNA in rodent tissues［J］. Neuroscience, 2002, 115(3): 645-656.

［3］ Bai ZhZh, Han ShF, Yang YZh, et al. Genetic cloning and expression of neuroglobin in high altitude hypoxic adaptation species-plateau pika(Ochotona curzoniae)［J］. Acta Theriologica Sinica, 2010, 30(1): 74-78.（in Chinese）

白振忠, 韩淑芬, 杨英忠等. 高原鼠兔脑红蛋白基因的克隆与组织表达［J］. 兽类学报, 2010, 30(1): 74-78.

［4］Zivin JA. Factors determining the therapeutic window for stroke ［J］. Neurology, 1998, 50(3): 599-603.

［5］Greenberg DA, Jin KN, Khan AA. Neuroglobin: an endogenous neuroprotectant［J］. Curr Opin Pharmacol, 2008, 8(1): 20-24.

［6］Sun Y, Jin K, Mao XO, et al. Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic-injury［J］. Proc Natl Acad Sci USA, 2001, 98(26): 15306-15311.［7］ Shang AJ, Zhou DB, Zhang ChG, et al.Cellular localization of neuroglobin expression in rat brain［J］. Chinese Journal of Neuromedicine, 2006, 5(9): 891-893.（in Chinese）

尚爱加, 周定标, 张成岗,等. 脑红蛋白在大鼠脑内表达的细胞定位［J］. 中华神经医学杂志, 2006, 5(9): 891-893. 

［8］Laufs T, Wystub S, Reuss S, et al. Neuron-specific expression of neuroglobin in mammals［J］. Neurosci Lett, 2004, 362(2): 83-86.

［9］Chen XQ, Qin LY, Zhang CG, et al. Presence of neuroglobin in cultured astrocytes［J］. Glia, 2005, 50(2): 182-186.

［10］Avivi A, Gerlach F, Joel A. Neroglobin, cytoglobin, and myoglobin contribute to hypoxia adaptation of the subterranean mole rat spalax［J］. Proc Natl Acad Sci USA, 2010, 107(50): 21570-21575.

［11］ Shang AJ, Zhou DB, Meng XH, et al. Distribution of neuroglobin in spinal code of adult rat［J］. Acta Anatomica Sinica, 2006, 37(5): 492-494.（in Chinese）

尚爱加, 周定标, 孟祥辉, 等. 脑红蛋白在成年大鼠脊髓中的分布［J］. 解剖学报, 2006, 37(5): 492-494.

［12］ Qin HJ, Zhang LSh, Guan P, et al. Study on the distribution and location of neuroglobin in human tissues and cells［J］. Journal of Sichuan University(Medical Science Edition), 2008, 39(4): 609-611.（in Chinese）

秦豪杰, 张录顺, 官鹏, 等. 脑红蛋白在人体组织细胞的定位分布及意义［J］. 四川大学学报(医学版), 2008, 39(4): 609-611.

［13］Schmidt M, Giessl A, Laufs T, et al. How does the eye breathe? Evidence for neuroglobin-mediated oxygen supply in the mammalian retina［J］. J Biol Chem, 2003, 278(3): 1932-1935.

［14］ Liao WG, Gao YQ, Cai MCh, et al. Effect of hypoxia on secretion of testosterone and expression of its synthesis relative protein and enzymes in rats［J］. Chinese Journal of Aerospace Medicine, 2006, 17(3): 191-195.（in Chinese）

廖卫公, 高钰琪, 蔡明春, 等. 低氧对雄性大鼠睾酮分泌及其合成相关蛋白和酶表达的影响［J］. 中华航空航天医学杂志, 2006, 17(3): 191-195. 