二苯乙烯苷通过调节组蛋白去乙酰化酶1水平拮抗沙鼠脑缺血/再灌注损伤

陈晓宇; 王栋; 刘晓利; 陈晓蓉; 李俊*

doi:10.3969/j.issn.0529-1356.2013.01.005

解剖学报 ›› 2013, Vol. 44 ›› Issue (1) : 25-29. DOI: 10.3969/j.issn.0529-1356.2013.01.005

神经生物学

二苯乙烯苷通过调节组蛋白去乙酰化酶1水平拮抗沙鼠脑缺血/再灌注损伤

陈晓宇¹,王栋¹,刘晓利¹,陈晓蓉¹,李俊^2*

作者信息 +

Role of tetrahydroxy stilbene glucoside on histone deacetylase 1 level in cerebral ischemic-reperfusion injury in gerbils

CHEN Xiao-yu¹,WANG Dong¹,LIU Xiao-li¹,CHEN Xiao-rong¹,LI Jun ^2*

Author information +

文章历史 +

摘要

目的探讨组蛋白去乙酰化酶1(HDAC1)水平改变在二苯乙烯苷（TSG）对沙鼠脑缺血/再灌注（I/R）损伤中的作用。方法 64只沙鼠随机分为假手术组、脑I/R模型对照组、TSG处理组和依达拉奉对照组。Morris水迷宫实验观察TSG对脑I/R鼠学习记忆功能的影响；神经功能评分、氯化三苯基四氮唑（TTC）染色观察TSG对经I/R脑组织的保护作用；免疫组织化学SP法观察脑组织中HDAC1蛋白的表达。Western blotting和RTPCR检测HDAC1蛋白和mRNA的水平。结果与模型组比较，TSG对I/R鼠的脑组织有保护作用，能够改善模型鼠学习记忆能力，使模型鼠脑组织HDAC1蛋白和mRNA水平明显升高。结论 TSG能够改善脑I/R损伤模型鼠认知功能，其作用机制与组蛋白乙酰化和去乙酰化动态平衡有关。

Abstract

bjective To investigate the role of tetrahydroxy stilbene glucoside(TSG) on histone deacetylase 1 (HDAC1) level in cerebral ischemic-reperfusion (I/R) injury in gerbils. Methods Sixty-four gerbils were randomly divided into four groups: sham group,model control group, TSG treatment group and the positive control medicine of edaravone injection group. The functions of learning and memory were detected by Morris water maze after 7 days. The cerebral infarction of protective action was investigated by nerve function score and triphenyltetrazolium chloride (TTC) staining. The protein and mRNA level of HDAC1 were detected by the immunohistochemical SP, Western blotting and RT-PCR methods. Results Compared with the model group, TSG had protective effect on ischemia reperfusion of the brain. TSG significantly enhanced the ability of learning and memory in I/R injury model gerbils. The protein expression and mRNA level of HDAC1 were significantly increased after TSG treatment in I/R injury model mice. Conclusion TSG has positively protective and therapeutic effect on cerebral I/R injury in gerbils. Its mechanism may be relevant with maintaining the homeostasis of histone acetylation and deacetylation.

导出引用

陈晓宇,王栋,刘晓利,陈晓蓉,李俊*. 二苯乙烯苷通过调节组蛋白去乙酰化酶1水平拮抗沙鼠脑缺血/再灌注损伤 [J]. 解剖学报. 2013, 44(1): 25-29 https://doi.org/10.3969/j.issn.0529-1356.2013.01.005

CHEN Xiao-yu,WANG Dong,LIU Xiao-li,CHEN Xiao-rong,LI Jun*. Role of tetrahydroxy stilbene glucoside on histone deacetylase 1 level in cerebral ischemic-reperfusion injury in gerbils[J]. Acta Anatomica Sinica. 2013, 44(1): 25-29 https://doi.org/10.3969/j.issn.0529-1356.2013.01.005

参考文献

[1] Cao Y, Mao X, Sun C,et al.Baicalin attenuates global cerebral ischemia/reperfusion injury in gerbils via anti-oxidative and anti-apoptotic pathways［J］.Brain Res Bull,2011, 85(6): 396-402.

［2］Hu SQ, Ye JS, Zong YY, et al. S-nitrosylation of mixed lineage kinase 3 contributes to its activation after cerebral ischemia［J］.J Biol Chem, 2012, 287(4):2364-2377. 

［3］ Wang D,Chen XY, Liu XL, et al. Effect of tetrahydroxystilbene glucoside on gerbils with cerebral ischemia-reperfusion injury［J］. Acta Universitatis Medicinalis Anhui, 2012,47(4)： 379-382.(in Chinese) 

王栋，陈晓宇，刘晓莉，等.二苯乙烯苷对沙鼠脑缺血/再灌注损伤的保护用及部分机制［J］.安徽医科大学学报，2012,47(4)： 379-382. 

［4］Yildirim F, Gertz K, Kronenberg G, et al. Inhibition of histone deacetylation protects wildtype but not gelsolin-deficient mice from ischemic brain injury［J］.Exp Neurol, 2008, 210(2): 531-542.

［5］Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats ［J］. Stroke, 1989,20 (1): 84-91.

［6］Tagliari B, Scherer EB, Machado FR,et al.Antioxidants prevent memory deficits provoked by chronic variable stress in rats［J］.Neurochem Res,2011, 36(12):2373-2380. ［7］Hou Y, Yang Q, Zhou L,et al.Tetrahydroxystilbene glucoside improves learning and (or) memory ability of aged rats and may be connected to the APP pathway［J］.Can J Physiol Pharmacol，2011，89(11):801-809.

［8］Baltan S, Bachleda A, Morrison RS, et al. Expression of histone deacetylases in cellular compartments of the mouse brain and the effects of ischemia［J］.Transl Stroke Res，2011，2(3):411-423.

［9］Gaub P, Tedeschi A, Puttagunta R, et al. HDAC inhibition promotes neuronal outgrowth and counteracts growth cone collapse through CBP/p300 and P/CAF-dependent p53 acetylation［J］.Cell Death Differ，2010，17(9):1392-1408. 

［10］Kim HJ, Leeds P, Chuang DM. The HDAC inhibitor, sodium butyrate, stimulates neurogenesis in the ischemic brain［J］. J Neurochem, 2009, 110 (4): 1226-1240. 

［11］Dash PK, Orsi SA, Moore AN. Histone deactylase inhibition combined with behavioral therapy enhances learning and memory following traumatic brain injury ［J］. Neuroscience, 2009, 163(1):1-8.