目的 探讨骨桥蛋白(OPN)在动情周期和妊娠早期小鼠输卵管的表达。方法 70只昆明雌性小白鼠分为动情周期组、妊娠组和假孕组，昆明雄性小白鼠15只用于与雌鼠合笼。取雌鼠动情周期、妊娠和假孕第1～5天的输卵管，利用免疫组织化学染色及半定量分析检测OPN在小鼠输卵管的定位和表达情况。结果 整个动情周期小鼠输卵管上皮组织OPN的免疫染色均呈阳性，动情期的输卵管上皮OPN染色的平均吸光度值（AEM>A/EM>）最高，动情间期组AA值最低；妊娠和假孕第1～5天小鼠输卵管上

Objective To investigate expression of osteopontin (OPN) in the mouse oviduct during the estrous cycle and early pregnancy. Methods A total of 70 female and 15 male mice of Kunming white strains were used in this study. Immunohistochemistry and image analysis were employed to detect and analyze the localization and expression of OPN in the mouse oviduct during the estrous cycle, pregnant and pseudopregnant from day 1 to 5, respectively. Results Throughout the estrous cycle, the OPN immunostaining of mouse oviduct epithelium was positive. The average absorbance (AEM>A/EM>) of OPN in oviduct epithelium was the highest in the estrus stage, and the lowest in the diestrus stage. The OPN immunostaining was positive throughout day 1 to 5 of pregnancy and pseudopregnancy. The expression of OPN reached peak on day 4 of pregnancy. There were significant differences in the same day between the pregnancy and the pseudopregnancy in the AEM>A/EM> value of OPN druing day 3 to 5 (EM>P /EM>< 0.05,EM> P/EM> < 0.01, EM>P/EM> < 0.05). Conclusion The expresses of OPN in the mouse oviduct epithelium may be regulated by estrogen during the estrous cycle, which is regulated by estrogen and preimplantation embryos during early pregnancy.