糖尿病对大鼠股骨头生长板分化和成骨的影响

陈海英; 陈小萍; 郑建盛; 陈少强; 刘文革; 唐军民

doi:10.3969/j.issn.0529-1356.2011.06.022

解剖学报 ›› 2011, Vol. 42 ›› Issue (6) : 825-831. DOI: 10.3969/j.issn.0529-1356.2011.06.022

组织学胚胎学发育生物学

糖尿病对大鼠股骨头生长板分化和成骨的影响

陈海英^{1*; 陈小萍^{1; 郑建盛^{1; 陈少强^{2; 刘文革^{3; 唐军民⁴}}}}}

作者信息 +

Effect of diabetes on the differentiation and osteogenesis mineralization offemoral head growth plate in rats

Author information +

文章历史 +

Abstract

Objective To study the effect of diabetes on development of long bones.Methods The diabetic model(STZ) of the rat was established.Rats were randomly divided into 3 normal groups：5-week(CON1), 10-week(CON2) and 15-week(CON3) and 3 diabetic groups：5-week(DM1), 10-week(DM2) and 15-week(DM3),10 rats each group. The change of collagen fiber depositing was observed by Van Gieson staining method. The microvascular density was measured by the ink infusion method.The techniques of in situ hybridization and RT-PCR were used in this study in order to analyze the intensity of vascular endothelial growth factor (VEGF)mRNA expression and content of decorin,respectively.Results The thickness and cellular density of the cell pillar in the growth plate were obviously lower in the 15-week diabetic group than those in the control group(EM>P/EM> <0.05).The content of collagen in the calcified zone of the growth plate was higher than that in the control group(EM>P/EM> <0.05),and the content of decorin decreased in the 15-week diabetic group(EM>P/EM> <0.01),the intensity of VEGFmRNA expression increased(EM>P/EM> <0.01),the microvascular density increased.Conclusion The cellular proliferation,differentiation and its osteogenesis mineralization in the growth plate of the femoral head in the 15-week diabetic rats a

导出引用

陈海英;陈小萍; 郑建盛; 陈少强; 刘文革; 唐军民. 糖尿病对大鼠股骨头生长板分化和成骨的影响[J]. 解剖学报. 2011, 42(6): 825-831 https://doi.org/10.3969/j.issn.0529-1356.2011.06.022

Effect of diabetes on the differentiation and osteogenesis mineralization offemoral head growth plate in rats[J]. Acta Anatomica Sinica. 2011, 42(6): 825-831 https://doi.org/10.3969/j.issn.0529-1356.2011.06.022

中图分类号： R587.2