LGR5在肝癌细胞及肝癌组织中的表达及意义

宋竹雯; 魏潇凡; 高建超; 白莉; 张宏权

doi:10.3969/j.issn.0529-1356.2011.06.017

解剖学报 ›› 2011, Vol. 42 ›› Issue (6) : 802-806. DOI: 10.3969/j.issn.0529-1356.2011.06.017

肿瘤生物学

LGR5在肝癌细胞及肝癌组织中的表达及意义

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Expression of LGR5 in hepatoma cells, HCC tissue and its significance

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Abstract

Objective To investigate the expression of LGR5 in human hepatocelluar cancer tissues and four human liver cancer cell lines and to evaluate its value in the development of hepatocellular cancer.Methods The mRNA and protein expression of LGR5 in human liver cancer cell lines and normal liver cell were detected by real-time PCR and Western blotting. Expression of LGR5 and β-catenin in hepatocellular cancer tissue was determined by immunohistochemistry. LGR5 localization and expression in HepG2 cells were tested by immunocytochemical staining. Results Western blotting and real-time PCR results demonstrated that the expression of LGR5 decreased significantly in HepG2, Hep3B, Huh7 and PLC cells compared to LO2 cells(EM>P/EM> <0.05). Immunohistochemistry results also showed that LGR5 was downregulated in cancer tissue compared to adjacent tumor tissue, whereas β-catenin expression was upregulated in cancer tissue. Conclusion Compared to adjacent tumor tissue and normal liver cells, LGR5 expression was significantly downregulated in 36 cases of hepatocelluar cancer tissues and four kinds of h

导出引用

宋竹雯;魏潇凡; 高建超;白莉;张宏权. LGR5在肝癌细胞及肝癌组织中的表达及意义[J]. 解剖学报. 2011, 42(6): 802-806 https://doi.org/10.3969/j.issn.0529-1356.2011.06.017

Expression of LGR5 in hepatoma cells, HCC tissue and its significance[J]. Acta Anatomica Sinica. 2011, 42(6): 802-806 https://doi.org/10.3969/j.issn.0529-1356.2011.06.017

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