17β-雌二醇对高同型半胱氨酸诱导人成骨肉瘤MG63细胞损伤的保护作用及机制

陈学秋; 戴纪男; 刘承杏; 边立功; 郭家智; 孙俊; 桂莉; 陆地

doi:10.3969/j.issn.0529-1356.2011.05.012

解剖学报 ›› 2011, Vol. 42 ›› Issue (5) : 635-639. DOI: 10.3969/j.issn.0529-1356.2011.05.012

细胞和分子生物学

17β-雌二醇对高同型半胱氨酸诱导人成骨肉瘤MG63细胞损伤的保护作用及机制

陈学秋^{1,2;戴纪男^{1;刘承杏^{1;边立功^{1;郭家智^{1;孙俊^{1;桂莉^{3*;陆地^1*}}}}}}}

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Protection effects and mechanism of 17β-estradiol in high homocysteine induced injury of human osteosarcoma MG63 cells EM>in vitro/EM>

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Abstract

Objective To explore the protection effects and molecular mechanisms of 17β-estradiol (17β-ESUB>2/SUB>) in high homocysteine (HHcy) induced injury of human osteosarcoma MG63 cells EM>in vitro/EM>. Methods Human osteosarcoma MG63 cells cultured EM>in vitro/EM> were treated with HHcy in different concentrations at different time after 17β-estradiol intervention treatment. The levels of reactive oxygen species (ROS ) were detected with a 2′,7′-dichloro-fluorescin diacetate probe (HSUB>2 /SUB>DCFDA ). The apoptotic and necrotic cells were determined by double nuclear staining with Hoechst 33342/PI. The expression levels of Caspase-3 and Bax were measured with Western blotting. Results 1.17β-ESUB>2/SUB>significantly suppressed the HHcy induced production of ROS in MG63 cells and the extent of suppression was positively associated with the dose and time. 2.17β-ESUB>2/SUB> significantly prevented MG63 cells from apoptosis or necrosis induced by HHcy and down-regulated t

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陈学秋;戴纪男;刘承杏;边立功;郭家智;孙俊;桂莉;陆地. 17β-雌二醇对高同型半胱氨酸诱导人成骨肉瘤MG63细胞损伤的保护作用及机制[J]. 解剖学报. 2011, 42(5): 635-639 https://doi.org/10.3969/j.issn.0529-1356.2011.05.012

Protection effects and mechanism of 17β-estradiol in high homocysteine induced injury of human osteosarcoma MG63 cells EM>in vitro/EM>[J]. Acta Anatomica Sinica. 2011, 42(5): 635-639 https://doi.org/10.3969/j.issn.0529-1356.2011.05.012

中图分类号： R322.7