干扰素-γ诱导大鼠肠黏膜微血管内皮细胞表达细胞黏附因子-1和干扰素-γ受体α

薛久洲; 崔永勇; 张涛; 刘树强; 段慧琴; 杨佐君; 胡格; 穆祥; 

doi:10.3969/j.issn.0529-1356.2011.05.010

解剖学报 ›› 2011, Vol. 42 ›› Issue (5) : 622-629. DOI: 10.3969/j.issn.0529-1356.2011.05.010

细胞和分子生物学

干扰素-γ诱导大鼠肠黏膜微血管内皮细胞表达细胞黏附因子-1和干扰素-γ受体α

薛久洲^{1,2;崔永勇^{1;张涛^{2;刘树强^{3;段慧琴^{1;杨佐君^{1;胡格^{1*;穆祥^1,2*}}}}}}}

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IFN-γ inducing ICAM-1 and IFN-γRα expression in cultured rat intestinal mucosa microvascular endothelial cells

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Abstract

Objective To investigate the influences of interferon-γ(IFN-γ) on the intercellular cell adhesion molecule-1(ICAM-1) and IFN-γRα expression in the cultured rat intestinal mucosa microvascular endothelial cells (RIMMVECs). Methods After the RIMMVECs separation culture, IFN-γ in different doses were used to induce the EM>in vitro/EM> cultured RIMMVECs, ICAM-1 and IFN-γRα expression in the cultured RIMMVECs at both the mRNA and protein levels which were detected by realtime PCR and flow cytometry, respectively. Results The results showed that RIMMVECs were activated by IFN-γ at the concentrations of 20μg/L and 40 μg/L. ICAM-1 expression increased in the activated RIMMVECs at both mRNA and protein levels, reaching peak effects at 6 hours; IFN-γRα mRNA expression was increased, but the protein decreased after stimulation by 20 μg/L; IFN-γRα mRNA expression peaked at 2 hours and then decreased after 6 hours when stimulated by

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薛久洲;崔永勇;张涛;刘树强;段慧琴;杨佐君;胡格;穆祥;. 干扰素-γ诱导大鼠肠黏膜微血管内皮细胞表达细胞黏附因子-1和干扰素-γ受体α[J]. 解剖学报. 2011, 42(5): 622-629 https://doi.org/10.3969/j.issn.0529-1356.2011.05.010

IFN-γ inducing ICAM-1 and IFN-γRα expression in cultured rat intestinal mucosa microvascular endothelial cells[J]. Acta Anatomica Sinica. 2011, 42(5): 622-629 https://doi.org/10.3969/j.issn.0529-1356.2011.05.010

中图分类号： S852