碱性成纤维细胞生长因子和环磷酸腺苷对神经前体细胞内源性端粒酶反转录酶基因转录水平的调控

张小燕; 王亚军; 刘胜勇; 王建伟; 陆爱丽; 王珂; 张卫光; 沈丽

doi:10.3969/j.issn.0529-1356.2011.04.010

解剖学报 ›› 2011, Vol. 42 ›› Issue (4) : 479-483. DOI: 10.3969/j.issn.0529-1356.2011.04.010

细胞分子生物学

碱性成纤维细胞生长因子和环磷酸腺苷对神经前体细胞内源性端粒酶反转录酶基因转录水平的调控

张小燕^{1; 王亚军^{1; 刘胜勇^{2;王建伟^{2; 陆爱丽^{1; 王珂^{2;张卫光^{2;沈丽^1*}}}}}}}

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Regulation of transcription level of endogenous human telomerase reverse transcriptase gene in neural progenitor cells by bFGF and cAMP

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Abstract

Objective Using hTERT-immortalized human neural progenitor cell line, hNPCs-G3, it was studied on the transcription activity of endogenous human telomerase reverse transcriptase(hTERT) gene and the mechanisms of proliferation and differentiation in the human neural progenitor cells. Methods Transient co-transfection of hTERT promoter-luciferase constructs and pSV-β-Gal control vector into HeLa cells and hNPCs-G3 cells, and detection of the transcription activities of different hTERT promoter-luciferase constructs. After co-tansfection as above, hNPCs-G3 cells were induced by bFGF, and the transcription activities of different length of hTERT promoters responsive to proliferation were analyzed. After the co-tansfection as above, hNPCs-G3 cells were induced by cAMP, and the transcription activities of different length of hTERT promoters responsive to differentiation were analyzed. Results The transcription activity of endogenous hTERT gene was low in human neural progenitor cells, and activity of the proximal promoter was the highest. bFGF could up-regulate the activitiy of endogenous hTERT gene in human neural progenitor cells, which mainly depended on the stretch of -2 098bp to -1 099bp and -3 216bp to -2 098bp upstream of ATG. cAMP could downregulate the transcription activity of endogenous hTERT gene in human neural progenitor cells, which inhibited the activity of proximal promoter.Conclusion The transcription activity of endogenous hTERT gene was low in human neural progeni

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张小燕;王亚军;刘胜勇;王建伟;陆爱丽;王珂;张卫光;沈丽. 碱性成纤维细胞生长因子和环磷酸腺苷对神经前体细胞内源性端粒酶反转录酶基因转录水平的调控[J]. 解剖学报. 2011, 42(4): 479-483 https://doi.org/10.3969/j.issn.0529-1356.2011.04.010

Regulation of transcription level of endogenous human telomerase reverse transcriptase gene in neural progenitor cells by bFGF and cAMP[J]. Acta Anatomica Sinica. 2011, 42(4): 479-483 https://doi.org/10.3969/j.issn.0529-1356.2011.04.010

中图分类号： R1394.2