何首乌饮对过度训练大鼠睾丸组织3β-羟基类固醇脱氢酶的影响

王凤威; 曲银娥; 赵秀军; 牛嗣云; 孙一翀; 陈雪; 郭凯华; 高福禄

doi:10.3969/j.issn.0529-1356.2011.03.020

解剖学报 ›› 2011, Vol. 42 ›› Issue (3) : 394-398. DOI: 10.3969/j.issn.0529-1356.2011.03.020

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何首乌饮对过度训练大鼠睾丸组织3β-羟基类固醇脱氢酶的影响

王凤威^{1;曲银娥^{2;赵秀军^{3;牛嗣云^{4;孙一翀^{1;陈雪^{5;郭凯华^{6;高福禄^7*}}}}}}}

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Effect of Heshouwuyin on the expression of 3β-hydroxysteroid dehydrogenase protein in testis tissue of overtrained rats

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Abstract

Objective To explore the effect of Heshouwuyin(HSWY) on the expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) protein in testis tissue of overtrained rats. Methods Thirty SD rats were randomized into 5 groups, including normal control group(A group), HSWY administered normal group(B group), model control group(C group), HSWY treated group (D group), HSWY beforehand-prevented group (E group), 6 animals in each group. The model of C, D and E group were made by overtraining, the D group was treated with HSWY by intragastric administration for 60 days after the success of model［20g/(kgI>&#/I>8226;d) including crude drug 9.6kg/L］, and the E group was prevented by administering HSWY before overtraining［20g/(kgI>&#/I>8226;d) including crude drug 96kg/L］. The density of testosterone was detected by radio-immunity, the expression of 3β-HSD was measured by immunohistochemistry, Western blotting and RT-PCR. Results Compared with C group, 3β-HSD protein was expressed in plasmid of leydig cells, and the expressions of 3β-HSD and 3β-HSD mRNA in D group and E group were increased(EM>P/EM>0.05) with no differenc

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王凤威;曲银娥;赵秀军;牛嗣云;孙一翀;陈雪;郭凯华;高福禄. 何首乌饮对过度训练大鼠睾丸组织3β-羟基类固醇脱氢酶的影响[J]. 解剖学报. 2011, 42(3): 394-398 https://doi.org/10.3969/j.issn.0529-1356.2011.03.020

Effect of Heshouwuyin on the expression of 3β-hydroxysteroid dehydrogenase protein in testis tissue of overtrained rats[J]. Acta Anatomica Sinica. 2011, 42(3): 394-398 https://doi.org/10.3969/j.issn.0529-1356.2011.03.020

中图分类号： R339.4