阿糖胞苷诱导肺腺癌细胞凋亡与p73激活相关

张露; 刘宁; 吕丹瑜; 马万云; 李英 

doi:10.3969/j.issn.0529-1356.2011.01.009

解剖学报 ›› 2011, Vol. 42 ›› Issue (1) : 50-53. DOI: 10.3969/j.issn.0529-1356.2011.01.009

论著

阿糖胞苷诱导肺腺癌细胞凋亡与p73激活相关

张露^{1 ;刘宁^{1 ;吕丹瑜^{1 ;马万云^{2 ;李英^{1 *}}}}}

作者信息 +

p73 activation is involved in regulation of Ara-C-induced apoptosis in human lung adenocarcinoma cells

Author information +

文章历史 +

Abstract

Objective To study the apoptosis pathway of human lung adenocarcinoma cell line A549 induced by 1-β-D-arabinofuranosylcytosine (Ara-C) EM>in vitro/EM>. Methods A549 cells were incubated with Ara-C for 72hours EM>in vitro/EM>. Biological changes of apoptotic cells were studied by TUNEL staining. Morphological changes of the A549 cells treated with Ara-C were observed by transmission electron microscope. The expressions of p53 and p73 were investigated by Western blotting. Results 1.Apoptotic rates of A549 cells exposure to Ara-C studied by TUNEL staining were higher than that of the control (EM>P/EM><0.01). 2.Apoptosis body was apparently observed by transmission electron microscope. 3.Endogenous p73 but not p53 was induced and activated in dose-dependent manner upon Ara-C treatment by Western blotting. Conclusion Ara-C can effectively induce apoptosis of A549 cells. DNA damage-induced apoptosis of A549 cells treated by Ara-C is independent of functional p53-Up-regulation of p73 may play an important role that enhances the sensitivity of A549 cells to Ara-C and be p

关键词

阿糖胞苷 / p73 / p53 / A549细胞 / TUNEL法 / 透射电镜 / 免疫印迹法

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

张露;刘宁;吕丹瑜 ;马万云;李英 . 阿糖胞苷诱导肺腺癌细胞凋亡与p73激活相关[J]. 解剖学报. 2011, 42(1): 50-53 https://doi.org/10.3969/j.issn.0529-1356.2011.01.009

p73 activation is involved in regulation of Ara-C-induced apoptosis in human lung adenocarcinoma cells[J]. Acta Anatomica Sinica. 2011, 42(1): 50-53 https://doi.org/10.3969/j.issn.0529-1356.2011.01.009

中图分类号： R734.2