音速波状蛋白与成纤维细胞生长因子-8联合诱导大鼠骨髓间充质干细胞分化为多巴胺能神经元

付文玉; 吕翠; 庄文欣; 陈丹丹; 吕娥; 王晓萃; 杨丽

doi:10.3969/j.issn.0529-1356.2011.01.004

解剖学报 ›› 2011, Vol. 42 ›› Issue (1) : 22-26. DOI: 10.3969/j.issn.0529-1356.2011.01.004

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音速波状蛋白与成纤维细胞生长因子-8联合诱导大鼠骨髓间充质干细胞分化为多巴胺能神经元

付文玉^{1*;吕翠^{1,2; 庄文欣^{1;陈丹丹^{1;吕娥^{1;王晓萃^1;杨丽¹}}}}}

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Differentiation of rat bone marrow mesenchymal stem cells into dopaminergic neurons after treatment with sonic hedgehog and fibroblast growth factor-8

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Abstract

Objective To explore the differentiation of rat bone marrow mesenchymal stem cells(BMSCs) into dopaminergic neurons EM>in vitro/EM> after induced by sonic hedgehog (SHH) and fibroblast growth factor-8 (FGF-8). Methods The BMSCs derived from rat bone marrow were cultured and passaged in vitro. After being induced by basic fibroblast growth factor(bFGF) for 24 hours, the BMSCs were incubated with SHH and FGF-8 for 12 days. Then the expressions of Nestin, neuron specific enolase(NSE), glia fibrillary acid protein(GFAP), tyrosine hydroxylase(TH), vesicular monoamine transporter-2(VMAT2), proliferating cell nuclear antigen(PCNA), Bcl-2, cyclindependent protein kinase2(CDK2) and Cyclin B1 were detected by immunocytochemical and immunofluorescence methods. Results Under the inverted microscope, the induced BMSCs showed typical neuronal morphological characteristics after induction, and they downregulated PCNA, CDK2 and Cyclin B1.The induced cells also expressed Nestin, NSE, TH and VMAT2 in high degree and GFAP in low degree. The percentages of induced cells and uninduced BMSCs positive for TH were (46

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付文玉;吕翠;庄文欣;陈丹丹;吕娥;王晓萃;杨丽. 音速波状蛋白与成纤维细胞生长因子-8联合诱导大鼠骨髓间充质干细胞分化为多巴胺能神经元[J]. 解剖学报. 2011, 42(1): 22-26 https://doi.org/10.3969/j.issn.0529-1356.2011.01.004

Differentiation of rat bone marrow mesenchymal stem cells into dopaminergic neurons after treatment with sonic hedgehog and fibroblast growth factor-8[J]. Acta Anatomica Sinica. 2011, 42(1): 22-26 https://doi.org/10.3969/j.issn.0529-1356.2011.01.004

中图分类号： R329.2