膜联蛋白A7低表达对人宫颈癌HeLa细胞系增殖与凋亡的影响

李欣; 陈雪; 王小杰; 路国兵; 高福禄 

doi:10.3969/j.issn.0529-1356.2010.04.017

解剖学报 ›› 2010, Vol. 41 ›› Issue (4) : 572-575. DOI: 10.3969/j.issn.0529-1356.2010.04.017

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膜联蛋白A7低表达对人宫颈癌HeLa细胞系增殖与凋亡的影响

李欣^{1;陈雪^{2 ;王小杰^{1 ;路国兵^{1; 高福禄^{2* }}}}}

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Influence of annexin A7 low expression on proliferation and apoptosis of HeLa cells

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Abstract

Objective To study whether annexin A7(ANXA7) low expression can affect apoptosis and proliferation of HeLa cells. Methods Western blotting was used to identify whether siRNA can highly surpress annexin A7 expressin. When the effect of the siRNA was assured, the siRNA was transfected into HeLa cells by lipofectamine 2000. At the same time, cells in negative control group were transfected negative siRNA while cells as blank control group did not receive any treatment. After transfection for 48 hours, resazurin assay was conducted and DNA content was analyzed by cytometry. Results Resazurin assay showed that proliferation of the siRNA interference cell group decreased significantly（EM>P/EM><0.05）. Cytometry test showed that apoptosis of the siRNA interference group increased obviously（EM>P/EM><0.05）. Conclusion ANXA7 low expression may a

导出引用

李欣;陈雪;王小杰;路国兵;高福禄 . 膜联蛋白A7低表达对人宫颈癌HeLa细胞系增殖与凋亡的影响[J]. 解剖学报. 2010, 41(4): 572-575 https://doi.org/10.3969/j.issn.0529-1356.2010.04.017

Influence of annexin A7 low expression on proliferation and apoptosis of HeLa cells[J]. Acta Anatomica Sinica. 2010, 41(4): 572-575 https://doi.org/10.3969/j.issn.0529-1356.2010.04.017

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