胰岛素促进体外兔角膜缘干细胞的增殖

苏敏; 肖莉; 许庭良

doi:10.3969/j.issn.0529-1356.2010.04.013

解剖学报 ›› 2010, Vol. 41 ›› Issue (4) : 550-553. DOI: 10.3969/j.issn.0529-1356.2010.04.013

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胰岛素促进体外兔角膜缘干细胞的增殖

苏敏^{1* ; 肖莉^{2 ;许庭良^1}}

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Insulin promotes the proliferation of rabbit limbal stem cells EM>in vitro/EM>

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Abstract

Objective To get limbal stem cells(LSCs)by primary culture and explore the influence of insulin on LSCs’proliferation. Methods The cells from the rabbit corneal limbal tissue were cultured and identified by immunohistochemical staining and transmission electron microscopy. The LSCs were treated with 5mg/L, 10mg/L, 20mg/L, 40mg/L, 80mg/L and 100mg/L insulin respectively. Then methyl thiazolyl tetrazolium(MTT)assay was used to explore the influence of insulin on LSCs’ proliferation. Results The experiment groups at the concentration of 5mg / L and 10mg / L had significantly higher cell viability than that of the control group（EM>P/EM>0.05）, and so were the 5mg/L group and 10mg/L group（EM>P/EM>0.05）. But there were no significant difference between the experimental groups at th

导出引用

苏敏;肖莉;许庭良. 胰岛素促进体外兔角膜缘干细胞的增殖[J]. 解剖学报. 2010, 41(4): 550-553 https://doi.org/10.3969/j.issn.0529-1356.2010.04.013

Insulin promotes the proliferation of rabbit limbal stem cells EM>in vitro/EM>[J]. Acta Anatomica Sinica. 2010, 41(4): 550-553 https://doi.org/10.3969/j.issn.0529-1356.2010.04.013

中图分类号： R329.24