磷脂酶C-三磷酸肌醇途径参与β-淀粉样肽（25-35）诱导的海马神经元［Ca 2+］i的增加

林智颖; 陈晓春; 张静; 林楠; 林凌; 林清; 王玮

doi:10.3969/j.issn.0529-1356.2010.04.002

解剖学报 ›› 2010, Vol. 41 ›› Issue (4) : 491-497. DOI: 10.3969/j.issn.0529-1356.2010.04.002

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磷脂酶C-三磷酸肌醇途径参与β-淀粉样肽（25-35）诱导的海马神经元［Ca ^{2+］i的增加}

林智颖^{1 ;陈晓春^{1* ; 张静^{1 ;林楠^{1 ;林凌^{2; 林清^{2 ;王玮^2}}}}}}

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Pathway of phospholipase C-inositol trisphosphate is involved in the increase of ［CaSUP>2+/SUP>］i induced by β-amyloid peptide (25-35) in hippocampal neurons

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Abstract

Objective To investigate the effect of endoplasmic reticulum(ER) calcium stores on calcium overload induced by β-amyloid peptide (25-35)(AβSUB>25-35/SUB>) in hippocampal neurons. Methods The hippocampal neurons were loaded with calcium-sensitive fluorescent indicator Fluo-3/AM.Intracellular calcium concentration (［CaSUP>2+/SUP>］i)changes were measured in different conditions of drug intervention by using laser scanning confocal microscopy. Results Groups of 2, 10, 20μmol/L AβSUB>25-35/SUB> all increased ［CaSUP>2+/SUP>］i in hippocampal neurons(EM>n/EM>=5, EM>P/EM><0.05). 2-APB, an inhibitor of ER CaSUP>2+/SUP> release through channels associated to inositol trisphosphate receptor(IP3R), was shown to prevent the aggregated AβSUB>25-35/SUB>induced rise of ［CaSUP>2+/SUP>］i, suggesting the involvement of CaSUP>2+/SUP>released by ER. However, dantrolene, an inhibitor of ER CaSUP>2+/SUP> release through channels associated to ryanodine receptor(RyR), could not prevent the rise of ［CaSUP>2+/SUP>］i induced by AβSUB>25-35/SUB>. Treatment with aggregated AβSUB>25-35/SUB> for 1 hour induced a significant decrease in the ER CaSUP>2+/SUP> content (EM>P/EM><0.01), which was more pronounced 24 hours after the addition of aggregated AβSUB>

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林智颖;陈晓春;张静;林楠;林凌;林清;王玮. 磷脂酶C-三磷酸肌醇途径参与β-淀粉样肽（25-35）诱导的海马神经元［Ca ^{2+］i的增加}[J]. 解剖学报. 2010, 41(4): 491-497 https://doi.org/10.3969/j.issn.0529-1356.2010.04.002

Pathway of phospholipase C-inositol trisphosphate is involved in the increase of ［CaSUP>2+/SUP>］i induced by β-amyloid peptide (25-35) in hippocampal neurons[J]. Acta Anatomica Sinica. 2010, 41(4): 491-497 https://doi.org/10.3969/j.issn.0529-1356.2010.04.002

中图分类号： R749.1SUP>+/SUP>5