Objective To study Liaoning Han nationality’s characteristics of digit ratio. Methods With informed consent, we randomly chose 728 (270 males and 458 females) healthy Liaoning Han people between 20 and 22 years old, excluding patients of endocrine and metabolic disease and the people whose fingers were malformed and injured. Their mothers had never taken any hormones during duration of pregnancy. We used direct measurement to measure digit length, SPSS 14.0 package to statistical analysis. Results Liaoning Han people’s digit length present 3D＞4D＞2D＞5D，female’s 2D＞male’s(EM>P/EM><0.001）、male’s 4D>female’s（EM>P/EM><0.001）;Digit ratio of Liaoning Han people possessed the tendency of 3D:5D＞4D:5D＞2D:5D＞3D:4D＞2D:4D＞2D:3D; Digit ratio of Liaoning Han nationality had sexual and bilateral differences, especially in 2D:4D; Digit ratio of Liaoning Han people was exceed the digit ratio of