表没食子儿茶素-3-没食子酸酯抑制小鼠皮肤外伤愈合组织基质金属蛋白酶1的表达

申羽佳; 李卫国; 张江江; 张惠; 袁红旭; 贾永芳; 王坤英

doi:10.3969/j.issn.0529-1356.2010.03.014

解剖学报 ›› 2010, Vol. 41 ›› Issue (03) : 395-399. DOI: 10.3969/j.issn.0529-1356.2010.03.014

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表没食子儿茶素-3-没食子酸酯抑制小鼠皮肤外伤愈合组织基质金属蛋白酶1的表达

申羽佳 ;李卫国*; 张江江 ;张惠; 袁红旭; 贾永芳 ;王坤英

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Epigallocatechin-3-gallate inhibits the expression of matrix metalloproteinase-1 in mouse cutaneous wound healing

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Abstract

Objective To explore the effects of epigallocatechin-3-gallate(EGCG) (50 mg/L) on the expression of MMP-1 mRNA and the levels of MMP-1 during mouse cutaneous wound healing. Methods Fifty-seven mice were divided into control, 50 mg/L EGCG and vehicle groups. The wound skins were prepared at 6, 12, 24, 72, 120 and 168 hours after injury, respectively. The expression of MMP-1 mRNA and levels of MMP-1 in wound healing skins were determined with reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Results It was found that 50 mg/L EGCG inhibits the expression of MMP-1 mRNA and decreases the levels of MMP-1 of the wound tissue at the late stage of wound healing (72 hours after skin injury). Conclusion 50 mg/L EGCG could inhibit the expression of MMP-1 mRNA and the MMP-1 production during cutaneous wound healing, a

导出引用

申羽佳;李卫国;张江江;张惠;袁红旭;贾永芳;王坤英. 表没食子儿茶素-3-没食子酸酯抑制小鼠皮肤外伤愈合组织基质金属蛋白酶1的表达[J]. 解剖学报. 2010, 41(03): 395-399 https://doi.org/10.3969/j.issn.0529-1356.2010.03.014

Epigallocatechin-3-gallate inhibits the expression of matrix metalloproteinase-1 in mouse cutaneous wound healing[J]. Acta Anatomica Sinica. 2010, 41(03): 395-399 https://doi.org/10.3969/j.issn.0529-1356.2010.03.014

中图分类号： Q249 Q786 R318