碱性成纤维细胞生长因子和表皮生长因子联合诱导骨髓基质细胞分化形成神经球

杨琴; 李琦; 曾志磊; 李傲; 谢鹏; 杨军; 任俊伟

doi:10.3969/j.issn.0529-1356.2010.03.002

解剖学报 ›› 2010, Vol. 41 ›› Issue (03) : 339-343. DOI: 10.3969/j.issn.0529-1356.2010.03.002

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碱性成纤维细胞生长因子和表皮生长因子联合诱导骨髓基质细胞分化形成神经球

杨琴^{1;李琦^{1;曾志磊^{2 ;李傲^{3 ;谢鹏^{1*;杨军^{1;任俊伟^1}}}}}}

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Differentiation of bone marrow stromal cells into neurospheres induced by basic fibroblast growth factor combined with epidermal growth factor in rats

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Abstract

P>Objective To observe whether bone marrow stromal cells(MSCs) can be induced to form neurospheres by basic fibroblast growth factor(bFGF) combined with epidermal growth factor (EGF) in rats. Methods MSCs were cultured with whole bone morrow. Effect of bFGF and EGF, inducing MSCs to differentiated into neurospheres was studied by morphology, immunofluorescence, and Western blotting. Results MSCs can be effectively induced to form neurospheres by bFGF and EGF.The neurospheres can passage and differentiated into neural cells.Immunofluorescence and Western blotting showed these neurospheres expressed nestin protein. Cells differentiated from neurospheres expressed marker proteins of neurons, glial cells and oligodendrocytes. Conclusion The results indicate that MSCs can be effectively induced to form neurospheres by bFGF and EGF. The neurospheres may amplify and differentiate into neurons, glial cells and oligodendrocytes./P>

关键词

骨髓基质细胞 / 碱性成纤维细胞生长因子 / 表皮生长因子 / 神经球 / 分化 / 免疫荧光 / 免疫印迹法 / 大鼠

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杨琴;李琦;曾志磊;李傲;谢鹏;杨军;任俊伟. 碱性成纤维细胞生长因子和表皮生长因子联合诱导骨髓基质细胞分化形成神经球[J]. 解剖学报. 2010, 41(03): 339-343 https://doi.org/10.3969/j.issn.0529-1356.2010.03.002

Differentiation of bone marrow stromal cells into neurospheres induced by basic fibroblast growth factor combined with epidermal growth factor in rats[J]. Acta Anatomica Sinica. 2010, 41(03): 339-343 https://doi.org/10.3969/j.issn.0529-1356.2010.03.002

中图分类号： R285.5