促甲状腺激素释放激素受体1和2在二甲磺酸乙烷处理大鼠睾丸中的表达及意义

朱华萍; 赵勇; 赵洁; 张远强    BR&gt;BR&gt;

doi:10.3969/j.issn.0529-1356.2010.02.017

解剖学报 ›› 2010, Vol. 41 ›› Issue (2) : 252-256. DOI: 10.3969/j.issn.0529-1356.2010.02.017

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促甲状腺激素释放激素受体1和2在二甲磺酸乙烷处理大鼠睾丸中的表达及意义

朱华萍^{1 ;赵勇^{2 ; 赵洁^{3 ;张远强^{3 *   BR>BR>}}}}

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P>Expression and significance of TRH-R1 and TRH-R2 in the testis of EDS-treated adult rats/P>

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Abstract

Objective To investigate the expression of thyrotropinreleasing hormone receptor (TRH-R) type1 and type2 in ethane dimethanesulphonate （EDS）treated rat testis, and to discuss the significance of its expression in Leydig cells. Methods To make the injured testis Leydig cells rat model with EDS treatment. Western blotting, immunohistochemical ABC and immunofluorescence double labeling methods were used to detect the expression and location of TRH-R1 and R2 in the testicular tissues of EDStreatedday 2,day 7,day 14,day 21 and day 28 rat mode, respectively. Results Western blotting results showed that the positive immunochemical staining was not found in the testicular tissues of the EDS-treated day 2 to day 14, on the other hand,they were found in EDS-treated-21 day and EDS-treated-28 day. Immunohistochemistry demonstrated that TRH-R1 and R2 expressed in the spindleshaped cells reappeared around seminiferous tubules of postEDS 21 days and 28 days groups. Immunofluorescence double labeling confirmed that these TRH-R1 and R2 positively stained cells were newly regenerated progenitor Leydig cells. Conclusion TRH-R1 and R2 ar

导出引用

朱华萍;赵勇;赵洁;张远强  . 促甲状腺激素释放激素受体1和2在二甲磺酸乙烷处理大鼠睾丸中的表达及意义[J]. 解剖学报. 2010, 41(2): 252-256 https://doi.org/10.3969/j.issn.0529-1356.2010.02.017

P>Expression and significance of TRH-R1 and TRH-R2 in the testis of EDS-treated adult rats/P>[J]. Acta Anatomica Sinica. 2010, 41(2): 252-256 https://doi.org/10.3969/j.issn.0529-1356.2010.02.017

中图分类号： R329