p16INK4A在动情周期和早孕期小鼠子宫内膜的表达

郗强; 朱兴春; 姜蓉; 杨戎; 魏莎莉; 张文文

doi:10.3969/j.issn.0529-1356.2010.01.026

解剖学报 ›› 2010, Vol. 41 ›› Issue (1) : 132-136. DOI: 10.3969/j.issn.0529-1356.2010.01.026

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p16^{INK4A在动情周期和早孕期小鼠子宫内膜的表达}

郗强^{1;朱兴春^{2;姜蓉^{3;杨戎^{1* ;魏莎莉^{1;张文文¹}}}}}

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Expression of p16SUP>INK4A /SUP>in mouse endometrium during the estrus cycle and the early pregnancy period

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Abstract

Objective To investigate the expression of tumor suppressor gene p16SUP>INK4A/SUP> in mouse endometrium during the estrus cycle and early pregnancy and its possible role in blastocyst implantation. Methods Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of p16INK4A mRNA,immunohistochemistry and Western blotting were applied to detect p16SUP>INK4A/SUP> protein in mouse endometrium tissues during the estrus cycle and early pregnancy.　 Results The intensity of p16SUP>INK4A/SUP> mRNA expression in mouse early pregnancy was higher than that in the estrus cycle.Compared with the other 3 stages, the level of p16SUP>INK4A/SUP> mRNA expression at estrus was obviously higher. During the early pregnancy, the level of p16SUP>INK4A/SUP> mRNA expression increased steadily from day 2 to day 5,reaching the maximal level on day 5,then decreasing. Both immunohistochemical and Western blotting analysis showed the same results in expression patterns of p16SUP>INK4A/SUP> protein for mouse endometrium tissues as those results of RT-PCR.Conclusion p16SUP>INK4A/SUP> is involved in the embryos penetrating into the endometrial barrier.

导出引用

郗强;朱兴春;姜蓉;杨戎;魏莎莉;张文文. p16^{INK4A在动情周期和早孕期小鼠子宫内膜的表达}[J]. 解剖学报. 2010, 41(1): 132-136 https://doi.org/10.3969/j.issn.0529-1356.2010.01.026

Expression of p16SUP>INK4A /SUP>in mouse endometrium during the estrus cycle and the early pregnancy period[J]. Acta Anatomica Sinica. 2010, 41(1): 132-136 https://doi.org/10.3969/j.issn.0529-1356.2010.01.026

中图分类号： R394