小鼠2-细胞胚胎卵裂球后代在囊胚中随机分布

王中伟; 岳顺利; 朱佳伟; 曹效彰; 孙琪; 王媛; 周佳勃

doi:10.3969/j.issn.0529-1356.2010.01.024

解剖学报 ›› 2010, Vol. 41 ›› Issue (1) : 124-127. DOI: 10.3969/j.issn.0529-1356.2010.01.024

论著

小鼠2-细胞胚胎卵裂球后代在囊胚中随机分布

王中伟;岳顺利;朱佳伟;曹效彰;孙琪;王媛;周佳勃*

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Progeny of 2-cell embryo blastomeres distribute in mouse blastocyst randomly

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Abstract

Objective Kunming strain(KM) mice were used as animal models. Nontoxic dextran conjugated with tetramethylrhodamine（TMR）and fluorecein isothiocyante（FITC）was microinjected to two of the 2-cell blastomere as molecular probe to trace the development fate of the blastomere ,in order to figure out the mechanisms of the formation of Em-Ab axis. Methods FITC- dextran was injected to zygote in order to make sure if it is noxious. Two blastomeres of 2-cell embryo were injected FITC- dextran and TMR- dextran respectively. Results When labeled embryo develeped to blastocyst, distribution of progeny of 2-cell embryo blastomeres can be detected.Conclusion The cells of blastomere randomly distributed either embryonic parts or extra

导出引用

王中伟;岳顺利;朱佳伟;曹效彰;孙琪;王媛;周佳勃. 小鼠2-细胞胚胎卵裂球后代在囊胚中随机分布[J]. 解剖学报. 2010, 41(1): 124-127 https://doi.org/10.3969/j.issn.0529-1356.2010.01.024

Progeny of 2-cell embryo blastomeres distribute in mouse blastocyst randomly[J]. Acta Anatomica Sinica. 2010, 41(1): 124-127 https://doi.org/10.3969/j.issn.0529-1356.2010.01.024

中图分类号： Q132.8