Grp78表达对人肝细胞癌细胞外信号调节激酶1/2活性和表达的影响

苏荣健; 李贞; 程留芳; 李宏丹; 魏嘉; 包翠芬

doi:10.3969/j.issn.0529-1356.2009.06.016

解剖学报 ›› 2009, Vol. 40 ›› Issue (6) : 928-932. DOI: 10.3969/j.issn.0529-1356.2009.06.016

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Grp78表达对人肝细胞癌细胞外信号调节激酶1/2活性和表达的影响

苏荣健^{1*; 李贞^{1,2; 程留芳^{2 ;李宏丹^{1 ;魏嘉^{1 ;包翠芬¹}}}}}

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Effect of Grp78 on the activation and expression of ERK 1/2 in human hepatocellular carcinoma tissues

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Abstract

Objective We examined the Grp78, ERK1/2 and phospho-ERK1/2 expressions in hepatocellular carcinoma(HCC) tissue samples EM>in vitro/EM>, we interfered the expression of Grp78 in SMMC-7721 cells to explore whether Grp78 is involved in ERK1/2 signal pathway. Methods The Grp78, ERK1/2 and phospho-ERK1/2 expressions were detected by immunohistochemistry and confirmed by Western blotting in 47 HCC tissue samples. The Grp78 expression in SMMC-7721 cells was interfered by plasmid transfection and siRNA, ERK1/2 phosphorylation and expression were determined by Western blotting. Results The Grp78 expression was significantly correlated with ERK1/2 and phospho-ERK1/2 in HCC tissue samples. Overexpression of Grp78 promoted ERK1/2 phosphorylation in SMMC-7721 cells and the increased ERK1/2 phosphorylation was inhibited by Grp78 knockdown. Conclusion Grp78 is involved in the regulation of ERK1/2 signal pathway and might be a potential target for the comprehensive therapy of HCC.

导出引用

苏荣健;李贞;程留芳;李宏丹;魏嘉;包翠芬. Grp78表达对人肝细胞癌细胞外信号调节激酶1/2活性和表达的影响[J]. 解剖学报. 2009, 40(6): 928-932 https://doi.org/10.3969/j.issn.0529-1356.2009.06.016

Effect of Grp78 on the activation and expression of ERK 1/2 in human hepatocellular carcinoma tissues[J]. Acta Anatomica Sinica. 2009, 40(6): 928-932 https://doi.org/10.3969/j.issn.0529-1356.2009.06.016

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