慢性强迫游泳应激抑郁模型大鼠行为学及海马Ca2+/钙调蛋白依赖性激酶Ⅱ的变化

王海涛; 刘昊; 徐爱军; 阚泉; 高俊玲

doi:10.3969/j.issn.0529-1356.2009.06.007

解剖学报 ›› 2009, Vol. 40 ›› Issue (6) : 881-885. DOI: 10.3969/j.issn.0529-1356.2009.06.007

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慢性强迫游泳应激抑郁模型大鼠行为学及海马Ca^{2+/钙调蛋白依赖性激酶Ⅱ的变化}

王海涛^{1;刘昊^{2; 徐爱军^{1;阚泉^{1;高俊玲^1*}}}}

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Changes of behavior, CaSUP>2+/SUP>/CaMKⅡ in hippocampus of rats with chronic forced swimming stress model

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Abstract

Objective To observe the changes of behavior, intracellular free calcium and the expression of calmodulin dependent protein kinase Ⅱ(CaMKⅡ) in the hippocampal neurons of chronic forced swimming stress rats. Methods Male Wistar rats were randomly divided into control group and chronic forced swimming stress group. The behavior was examined using sucrose preference test, open-filed test and Morris water maze. The intracellular free calcium was examined by fluorescence spectrophotometer. The expression of CaMKⅡ was detected using colloidal gold immunoelectron microscopy technique, Western blotting and RT-PCR. Results The consumption of sucrose and erect quantity of chronic forced swimming stress group were lower than those of control group（EM>P/EM><0.01, EM>P/EM><0.05）. The escape latency time in Morries water maze test of chronic forced swimming stress group was higher than that of control group（EM>P/EM><0.01）. The intracellular free calcium level and the expression of CaMKⅡ in the hippocampus was higher than that of control group（EM>P/EM><0.01）.Conclusion The lasting dys

导出引用

王海涛;刘昊;徐爱军;阚泉;高俊玲. 慢性强迫游泳应激抑郁模型大鼠行为学及海马Ca^{2+/钙调蛋白依赖性激酶Ⅱ的变化}[J]. 解剖学报. 2009, 40(6): 881-885 https://doi.org/10.3969/j.issn.0529-1356.2009.06.007

Changes of behavior, CaSUP>2+/SUP>/CaMKⅡ in hippocampus of rats with chronic forced swimming stress model[J]. Acta Anatomica Sinica. 2009, 40(6): 881-885 https://doi.org/10.3969/j.issn.0529-1356.2009.06.007

中图分类号： R749.4