两种显示大鼠不同组织微血管方法的比较

张爽; 张涛; 王磊; 王安如; 俞英昉; 滕可导

doi:10.3969/j.issn.0529-1356.2009.05.031

解剖学报 ›› 2009, Vol. 40 ›› Issue (5) : 840-843. DOI: 10.3969/j.issn.0529-1356.2009.05.031

技术方法

两种显示大鼠不同组织微血管方法的比较

张爽^{1,2; 张涛^{3 ;王磊^{1; 王安如^{1; 俞英昉^{1; 滕可导^1*}}}}}

作者信息 +

Comparison between two methods for displaying microvessels in rat different tissues

Author information +

文章历史 +

Abstract

bjective To investigate the differences between CD34 immunohistochemistry and NBT/BCIP enzymic histochemistry in displaying microvessels of various tissues. Methods The microvessels in rat small intestinal, kidney, spleen, liver, heart, lung and skin were stained by CD34 immunohistochemistry and NBT/BCIP enzymic histochemistry, and the two methods were compared by morphological analysis and microscopic count. Results We found that the microvessels in all selected tissues above mentioned were stained by CD34 immunohistochemistry, and that the microvessels in small intestinal, kidney, liver and heart tissues, but not in spleen, lung and skin tissues, were stained by NBT/BCIP enzymic histochemistry. And in the same sections of one tissue, the microvascular density by NBT/BCIP enzymic histochemistry was higher than that by CD34 immunohistochemistry. Conclusion CD34 immunohistochemistry is generally suitable to display the microvesssels in various organs, while NBT/BCIP enzymic histochemistry shows some organ microvessels at higher density.

导出引用

张爽;张涛;王磊;王安如;俞英昉;滕可导. 两种显示大鼠不同组织微血管方法的比较[J]. 解剖学报. 2009, 40(5): 840-843 https://doi.org/10.3969/j.issn.0529-1356.2009.05.031

Comparison between two methods for displaying microvessels in rat different tissues[J]. Acta Anatomica Sinica. 2009, 40(5): 840-843 https://doi.org/10.3969/j.issn.0529-1356.2009.05.031

中图分类号： R329.3 S852.16