Objective To provide sectional anatomical basis for clinical magnetic resonance imaging (MRI) diagnosis of the interosseous talocalcaneal ligament. Methods Respectively,twelve cadaveric right feet taped in 20°plantarflexion position were frozen and cut into 3-mm-thick slices in coronal(EM>n/EM>=4),sagittal(EM>n/EM>=4) and transverse(EM>n/EM>=4) planes.Then we compared the slices with the MR images. Results In sagittal section through lateral border of collum tali, interosseous talocalcaneal ligament is within (19.2±2.8)mm. In coronal section through medial part of corpus tali, interosseous talocalcaneal ligament is within (17.9±5.2)mm.In the sagittal section through medial part of corpus tail, interosseous talocalcaneal ligament can be displayed most distinctly and it is 17.2mm long,11.9mm wide in sagittal diameter. In coronal section through medial part of corpus tali, interosseous talocalcaneal ligament can be displayed most distinctly and it is 13.1mm long,14.1mm wide in transverse diameter. In transverse section,the interosseous talocalcaneal ligament can not be displayed clearly.Conclusion MRI provides excellent delineation of the interosseous talocalcaneal ligament and evidence for the diagnosis of the interosseous talocalcaneal ligame