孕酮调节小鼠脾脏CD11c阳性树突状细胞白细胞介素和TNF-α的表达

杨林松; 冯敏敏; 周正球; 侯亚义

doi:10.3969/j.issn.0529-1356.2009.05.012

解剖学报 ›› 2009, Vol. 40 ›› Issue (5) : 755-759. DOI: 10.3969/j.issn.0529-1356.2009.05.012

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孕酮调节小鼠脾脏CD11c阳性树突状细胞白细胞介素和TNF-α的表达

杨林松^{1*; 冯敏敏^{1 ;周正球^{2; 侯亚义³}}}

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Regulation of progesterone on interleukins and TNF-α expression ofmurine CD11c positive splenic dendritic cells

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Abstract

Objective To elucidate the effect of progesterone (Prog) on the cytokine expressions of murine CD11c positive splenic dendritic cells (SDCs). Methods SDCs were purified by immune beads and treated with various concentrations of Prog for 24hours, then cytokine expressions of SDCs in the mRNA level were analyzed through reverse transcription-polymerase chain reaction (RT-PCR). Results Prog increased the transcript of IL-6 in middle dose group but decreased it in high dose, and the level of IL-10 was increased in low and middle dose groups while decreased in high dose group. Furthermore, the transcripts of IL-12 and TNF-α were increased and decreased respectively in all Prog treated groups.Conclusion Prog might regulate organ immune response through influencing cytokine expressions of SDCs. Low- and middle-dose Prog switch organ immune response to Th2-type, while high-dose Prog develops a Th1-type immune response.

导出引用

杨林松;冯敏敏;周正球;侯亚义. 孕酮调节小鼠脾脏CD11c阳性树突状细胞白细胞介素和TNF-α的表达[J]. 解剖学报. 2009, 40(5): 755-759 https://doi.org/10.3969/j.issn.0529-1356.2009.05.012

Regulation of progesterone on interleukins and TNF-α expression ofmurine CD11c positive splenic dendritic cells[J]. Acta Anatomica Sinica. 2009, 40(5): 755-759 https://doi.org/10.3969/j.issn.0529-1356.2009.05.012

中图分类号： R392.12