小RNA干扰Akt2对U87恶性胶质瘤细胞迁移和侵袭能力的影响

张宝刚; 谷峰; 李文良; 郭华; 张宁; 马勇杰

doi:10.3969/j.issn.0529-1356.2009.05.001

解剖学报 ›› 2009, Vol. 40 ›› Issue (5) : 691-695. DOI: 10.3969/j.issn.0529-1356.2009.05.001

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小RNA干扰Akt2对U87恶性胶质瘤细胞迁移和侵袭能力的影响

张宝刚^{1,4; 谷峰^{2 ;李文良^{3; 郭华^{1;张宁^{1;马勇杰^1*}}}}}

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EM>In vitro/EM> study of Akt2 function on U87 malignant glioma cells migration and invasion

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Abstract

Objective To detect the effect of Akt2 on U87 glioblastoma cells migration and invasion by Akt2 small RNA interference technology EM>in vitro/EM>.Methods Akt2 expression was knocked down in U87 glioblastoma cells by Akt2 siRNA, stable clones were used to perform wound healing assay, F-actin polymerization assay, adhesion assay and matrigel invasion assay. Western blotting was used to check the expression level of phosphorylated cofilin and LIMK. Results Result of wound healing assay suggested that motility of U87 cells was significantly decreased with deficiency of Akt2; U87 cells with Akt2 deficiency was not capable of assembling actin effectively compared to control cells by F-actin polymerization assay. Further, phosphorylated cofilin and LIMK expression which are important for cellular motility were all decreased with Akt2 deficiency. Besides, adhesion and invasion ability of U87 cells were significantly impaired after Akt2 was knocked down. Conclusion Akt2 is important for U87 cells migration and invasion. Akt2 deficiency will lead to impairment of U87 glioblastoma cells motility, adhesive and invasive ability EM>in vitro/EM>.

导出引用

张宝刚;谷峰;李文良;郭华;张宁;马勇杰. 小RNA干扰Akt2对U87恶性胶质瘤细胞迁移和侵袭能力的影响[J]. 解剖学报. 2009, 40(5): 691-695 https://doi.org/10.3969/j.issn.0529-1356.2009.05.001

EM>In vitro/EM> study of Akt2 function on U87 malignant glioma cells migration and invasion[J]. Acta Anatomica Sinica. 2009, 40(5): 691-695 https://doi.org/10.3969/j.issn.0529-1356.2009.05.001

中图分类号： R739.41