羧基荧光素乙酰乙酸与四甲基偶氮唑盐法检测人脐血间充质干细胞刺激脾淋巴细胞增殖作用的比较

孙海梅; 季凤清; 王屹; 王丹妮; 赵春礼; 杨慧

doi:10.3969/j.issn.0529-1356.2009.04.032

解剖学报 ›› 2009, Vol. 40 ›› Issue (4) : 680-684. DOI: 10.3969/j.issn.0529-1356.2009.04.032

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羧基荧光素乙酰乙酸与四甲基偶氮唑盐法检测人脐血间充质干细胞刺激脾淋巴细胞增殖作用的比较

孙海梅^{1; 季凤清^{1* ;王屹^{3 ;王丹妮^{1; 赵春礼^{2 ;杨慧²}}}}}

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Compararative investigation of proliferation of lymphocytes stimulated by human umbilical cord blood mesenchymal stem cells with CFSE and MTT

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Abstract

Objective To compare the sensitivities of the stimulation effect of human cord blood mesenchymal stem cells(CB-MSCs) and CB-MSCs of neuronal differentiation to lymphocytes(LCs) detected with carboxyfluorescein didcetate(CFSE) and MTT. Methods To prepare LCs from SD rat and divided into four group stimulating cells: 1. CB-MSCs; 2. Dif-CB-HSCs; 3. SH-SY5Y（positive control）; 4. Auto-LC（negative control）.Stimulating cells were respectively Co-cultured with LCs. The proliferation of LCs was detected with MTT and CFSE (EM>n/EM>=3). Results CB-MSCs and Dif-CB-HSCs stimulated LCs to proliferate more weakly than positive control detected with MTT and CFSE. The quantity of proliferation of lymphocytes Co-cultured with CB-MSCs and Dif-CB-HSCs were higher than that of Auto-LC detected with CFSE. But MTT OD value of CB-MSCs and Dif-CB-HSCs was a little lower than that of Auto-LC. Statistical analysis results showed no significant difference.Conclusion CFSE can reflect proliferation status of lymphocytes better than MTT. CFSE shows mor

导出引用

孙海梅;季凤清;王屹;王丹妮;赵春礼;杨慧. 羧基荧光素乙酰乙酸与四甲基偶氮唑盐法检测人脐血间充质干细胞刺激脾淋巴细胞增殖作用的比较[J]. 解剖学报. 2009, 40(4): 680-684 https://doi.org/10.3969/j.issn.0529-1356.2009.04.032

Compararative investigation of proliferation of lymphocytes stimulated by human umbilical cord blood mesenchymal stem cells with CFSE and MTT[J]. Acta Anatomica Sinica. 2009, 40(4): 680-684 https://doi.org/10.3969/j.issn.0529-1356.2009.04.032

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