乙醇对大鼠脑内5-HT能神经体系的影响

李双成; 康云霄; 石葛明; 崔慧先; 王磊; 李学平

doi:10.3969/j.issn.0529-1356.2009.03.010

解剖学报 ›› 2009, Vol. 40 ›› Issue (3) : 395-400. DOI: 10.3969/j.issn.0529-1356.2009.03.010

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乙醇对大鼠脑内5-HT能神经体系的影响

李双成^{1,2; 康云霄^{1 ;石葛明^{1* ;崔慧先^{2 ;王磊^{2; 李学平^1}}}}}

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Effect of ethanol on the serotonergic systems in rat brain

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Abstract

Objective To study the effect of ethonal on the serotonergic systems by analyzing the altered expression of tryptophan hydroxylase (TPH), serotonin (5-HT) and serotonin transporter (SERT) in the brain of ethanoltreated rats. Methods Wistar rats were treated with 20% ethanol for 6 months. Immunocytochemistry, flow cytometry and Western blotting were used to analyze the altered expression of the serotonergic markers in different brain regions of the ethanoltreated rats. Results 1. Immunohistochemistry showed the number of TPH or 5-HT positive neurons in dorsal raphe nucleus (DRN) is less in ethanoltreated rats than that in control (EM>P/EM>0.01), the diameter of TPH neurons in DRN is smaller than that in control (EM>P/EM>0.01) and the mean gray value of TPH, 5-HT and SERT in observed regions of the ethanoltreated rats is larger than that in control (EM>P/EM>0.05). 2. Flow cytometry showed the expression of TPH, 5-HT and SERT in the studied regions of the ethanoltreated rats decreased significantly compared with control (EM>P/EM>0.05). 3. Western blotting showed the ratio of SERT/β-actinin or TPH/β-actinin in analyzed regions of the ethanol-treated

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李双成;康云霄;石葛明 ;崔慧先;王磊;李学平. 乙醇对大鼠脑内5-HT能神经体系的影响[J]. 解剖学报. 2009, 40(3): 395-400 https://doi.org/10.3969/j.issn.0529-1356.2009.03.010

Effect of ethanol on the serotonergic systems in rat brain[J]. Acta Anatomica Sinica. 2009, 40(3): 395-400 https://doi.org/10.3969/j.issn.0529-1356.2009.03.010

中图分类号： Q952.4