利用亮甲酚蓝染色筛选优质猪卵母细胞

田江天; 王振坤; 郑重; 孙爽; 徐萌; 刘忠华

doi:10.3969/j.issn.0529-1356.2009.02.035

解剖学报 ›› 2009, Vol. 40 ›› Issue (2) : 340-343. DOI: 10.3969/j.issn.0529-1356.2009.02.035

技术方法

利用亮甲酚蓝染色筛选优质猪卵母细胞

田江天^{1;王振坤^{1 ;郑重^{1;孙爽^{1;徐萌^{2;刘忠华^1*}}}}}

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Selection of developmentally competent pig oocytes using brilliant cresyl blue staining

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Abstract

P>Objective To enhance efficiency of EM>in vitro/EM> embryos culture system of pig using brilliant cresyl blue (BCB) stain. Methods To stain the cumulusoocyte complexes through BCB then evaluate oocytes maturation rates and developmental competence of early embryos. Results The percentage (91.6%) of the MⅡ stage oocytes of pigs was higher in the BCBcolorable group (BCBSUP>+/SUP> ) than those (64.0%) in the noncolorable (BCBSUP>－/SUP>) and control (77.5%) groups (EM>P/EM><0.05). The pig parthenogenetic blastocyst rate (34.9%) was significantly higher in the BCB+ group than those in the control and BCBSUP>－/SUP> groups, (23% and 9.5%) respectively (EM>P/EM><0.05). The rate (23.9%) of pig somatic cell nucleus-transferred embryos into blastocyst was significantly higher in the BCBSUP>+/SUP> group than those in the control and the BCB

导出引用

田江天;王振坤;郑重;孙爽;徐萌;刘忠华. 利用亮甲酚蓝染色筛选优质猪卵母细胞[J]. 解剖学报. 2009, 40(2): 340-343 https://doi.org/10.3969/j.issn.0529-1356.2009.02.035

Selection of developmentally competent pig oocytes using brilliant cresyl blue staining[J]. Acta Anatomica Sinica. 2009, 40(2): 340-343 https://doi.org/10.3969/j.issn.0529-1356.2009.02.035

中图分类号： Q331