乙醛脱氢酶2抑制剂大豆甙对缺氧心肌细胞的MAPK信号转导作用

徐丹令; 孙爱军; 王时俊; 王翔飞; 贾剑国; 顾兴华; 王克强; 邹云增; 葛均波

doi:10.3969/j.issn.0529-1356.2009.02.017

解剖学报 ›› 2009, Vol. 40 ›› Issue (2) : 256-259. DOI: 10.3969/j.issn.0529-1356.2009.02.017

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乙醛脱氢酶2抑制剂大豆甙对缺氧心肌细胞的MAPK信号转导作用

徐丹令^{1，2 ;孙爱军^{1 ;王时俊^{1 ;王翔飞^{1 ;贾剑国^{1;顾兴华^{1 ;王克强^{1 ;邹云增^{1 ;葛均波^1*}}}}}}}}

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Study of ALDH2 inhibitor daidzin role on rat myocytes MAPK singaling pathway under hypoxia

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Abstract

Objective To observe the role of ALDH2 on cardiac myocytes variation of reactive oxygen species (ROS), apoptosis and mitogen-activated protein kinases (MAPK) signal route under hypoxia. Methods Cultured cardiomyocytes of neonatal rats were used. Hypoxia was imposed to the cardiomyocytes with or without daidzin pretreatment. ROS was measured by C400, apoptosis by the DeadEndSUP>TM/SUP>TUNEL system and MAPK signal pathway by Western blotting. Each group was dealt with more than three times. Results Compared to hypoxia group, that aldehyde dehydrogenase2(ALDH2) specifically inhibited by pretreated daidzin had a high frequency of ROS, apoptosis and an increased activation of MAPK signal enzymes including phosphorylated ERK1/2, JNK and P38Conclusion The cardiac myocytes variation of ROS, apoptosis and MAPK signal path resulting from hypoxia had a close relation with ALDH2 Myocytes showed decreased tolerance to hypoxia after ALDH2 specifically inhibited by daidzin. ALDH2 prevents hypoxia myocardial in

导出引用

徐丹令;孙爱军;王时俊;王翔飞;贾剑国;顾兴华;王克强;邹云增;葛均波. 乙醛脱氢酶2抑制剂大豆甙对缺氧心肌细胞的MAPK信号转导作用[J]. 解剖学报. 2009, 40(2): 256-259 https://doi.org/10.3969/j.issn.0529-1356.2009.02.017

Study of ALDH2 inhibitor daidzin role on rat myocytes MAPK singaling pathway under hypoxia[J]. Acta Anatomica Sinica. 2009, 40(2): 256-259 https://doi.org/10.3969/j.issn.0529-1356.2009.02.017

中图分类号： R363