Smad7和组蛋白去乙酰化酶1在小鼠马兜铃酸肾病中的表达

彭晓兰; 董晓凯; 廖芳芳; 高磊; 张中文; 吴国娟

doi:10.3969/j.issn.0529-13562012.01.017

解剖学报 ›› 2012, Vol. 43 ›› Issue (1) : 88-92. DOI: 10.3969/j.issn.0529-13562012.01.017

组织学胚胎学与发育生物学

Smad7和组蛋白去乙酰化酶1在小鼠马兜铃酸肾病中的表达

彭晓兰; 董晓凯; 廖芳芳; 高磊; 张中文^{*; 吴国娟^*}

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Expression of Smad7 and histone deacetylase 1 in the mouse with aristolochic acid nephropathy

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Abstract

Objective To investigate the roles of Smad7 and histone deacetylase 1(HDAC1) in the development of aristolochic acid nephropathy (AAN), we studied their expressions in AAN. Methods The expressions of Smad7 and HDAC1 in the AAN and control groups were measured by immunohistochemical technique, Western blotting and RT-PCR. A total of 32 male mice were randomized into four groups. Aristolochic acid(AA)Ⅰ was dissolved in PBS and was given to mice in three treatment groups through intragastric administration ［4 mg/（kgI>&#/I>8226;d）, 8 mg/（kgI>&#/I>8226;d）, and 16 mg/（kgI>&#/I>8226;d）,respectively］. Results The immunohistochemical positive reaction for HDAC1 was distributed in the epithelial cell nuclei of the renal tubules. The expression level of HDAC1 was significantly higher in the AAN group than that in the control group. The results of Western blotting and RT-PCR showed that the protein expression of Smad7 in the AAN group was significantly lower than that of the control group, while the situation was opposite for HDACl expression. Conclusion

导出引用

彭晓兰;董晓凯;廖芳芳;高磊;张中文;吴国娟. Smad7和组蛋白去乙酰化酶1在小鼠马兜铃酸肾病中的表达[J]. 解剖学报. 2012, 43(1): 88-92 https://doi.org/10.3969/j.issn.0529-13562012.01.017

Expression of Smad7 and histone deacetylase 1 in the mouse with aristolochic acid nephropathy[J]. Acta Anatomica Sinica. 2012, 43(1): 88-92 https://doi.org/10.3969/j.issn.0529-13562012.01.017

中图分类号： R965.1