目的 探讨P-选择素（CD62P）在阿霉素（DOX）诱导的人心肌细胞系 AC16 和人心力衰竭心房肌组织中的表达变化。方法体外培养 AC16，通过不同浓度盐酸阿霉素（1、5、15 μmol//L ）处理构建不同程度心肌损伤细胞模型。实验设置对照组（Ctrl）和损伤模型组（IM），分为轻度（IM-Mi）、中度（IM-Mo）、重度（IM-S）组。采用免疫荧光染色鉴定 AC16 细胞表型，CCK-8 法检测 AC16 细胞增殖活性，Western blotting及免疫组织化学染色分别检测 AC16 细胞系和人心衰组织样本中 CD62P 蛋白表达，酶联免疫吸附法测定 DOX 诱导的 AC16 细胞上清和心衰患者血清中 CD62P 的表达水平。结果CD62P 在 AC16 细胞系中随 DOX 浓度升高，损伤程度加重，表达水平升高（P<0.05），呈现剂量依赖性升高趋势。人心衰组织中CD62P阳性率显著高于对照组，且随心衰程度加重表达水平升高（P<0.05)，CD62P 血清检测结果与组织结果表达趋势一致。结论 CD62P 在局部组织和外周血清的表达水平与心肌损伤程度成正相关，提示其无论外周还是局部组织的表达水平均可作为评估心衰进展的潜在生物标志物。

Objective To investigate the expression changes of P-selectin（CD62P）in the doxorubicin （DOX）induced damaged cardiomyocyte line AC16 and atrial myocardial tissue from patients with heart failure(HF). Methods The AC16 cardiomyocyte line was cultured in vitro, and cardiomyocyte injury models with different degrees of damage were established using DOX at concentrations of 1μmol/L, 5μmol/L, and 15μmol/L. The experiment included control group (Ctrl) and myocardial injury model group (IM), which was further divided into mild (IM-Mi), moderate (IM-Mo), and severe (IM-S) subgroups. Immunofluorescence staining (IF) was used to identify the phenotype of AC16 cells, and the CCK-8 assay was employed to detect the proliferative activity of AC16 cells. Western blotting and immunohistochemical (IHC)staining were respectively used to determine the protein expression of CD62P in the AC16 cell line and human HF tissue samples. Additionally, ELISA was conducted to measure the expression levels of CD62P in the supernatant of AC16 cells with different degrees of DOX-induced damage and in the serum of patients with HF. Results In the AC16 cell line, the expression level of CD62P increased with the elevation of DOX concentration （P<0.05）, showing a dose-dependent upward trend. The positive rate of CD62P in the human HF group was significantly higher than that in the control group; Moreover, the expression level of CD62P increased as the severity of HF worsened (P<0.05). The detection result of CD62P in serum were consistent with the expression trend of CD62P in tissue. Conclusion The expression levels of CD62P in local tissues and peripheral serum are positively correlated with the degree of myocardial injury, suggesting that CD62P may serve as a potential biomarker for evaluating the progression of heart failure.