低葡萄糖诱导少突胶质前体细胞中单核羧酸转运体1的胞质转运

doi:10.16098/j.issn.0529-1356.2026.03.005

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解剖学报 ›› 2026, Vol. 57 ›› Issue (3) : 306-312. DOI: 10.16098/j.issn.0529-1356.2026.03.005

肿瘤生物学

低葡萄糖诱导少突胶质前体细胞中单核羧酸转运体1的胞质转运

张优利¹ 周鹏² 张晓艳^1*

作者信息 +

Cytoplasmic transport of monocarboxylate transporter 1 in oligodendrocyte precursor cells induced by low glucose

ZHANG You-li¹, ZHOU Peng², ZHANG Xiao-yan^1*

Author information +

文章历史 +

摘要

目的探讨低葡萄糖条件下早期少突胶质前体细胞（OPCs）中单核羧酸转运体1（MCT1）从胞质转运至细胞膜的机制。方法通过低葡萄糖培养基处理OPCs，环磷酸腺苷（cAMP）化学发光免疫分析试剂盒检测细胞内cAMP表达水平，Western blotting检测钠钾三磷酸腺苷酶（NaK-ATPase）、MCT1的表达水平，流式细胞术检测线粒体膜电位及蛋白质质谱检测MCT1磷酸化状态，并通过免疫荧光染色观察低葡萄糖条件下或添加cAMP激动剂福司柯林（Fors）时MCT1与网格蛋白（clathrin）、小窝蛋白1（caveolin-1）的共定位情况，最后验证丙酮酸钠（SP）是否可上调MCT1表达。结果低葡萄糖环境诱导OPCs发生显著的应激反应，且在该环境下MCT1的磷酸化能够增强其与网格蛋白的相互作用。此外，MCT1的转运过程依赖于cAMP信号参与，而SP可上调MCT1的表达。结论低葡萄糖条件下，cAMP与网格蛋白可协助磷酸化的MCT1从胞质转运至细胞膜。

Abstract

Objective To investigate the mechanism underlying the translocation of monocarboxylate transporter 1 (MCT1) from the cytoplasm to the plasma membrane in oligodendrocyte precursor cells (OPCs) under early low-glucose conditions. Methods OPCs were cultured in low-glucose medium. Intracellular cyclic adenosine monophosphate (cAMP) levels were quantified using a chemiluminescence immunoassay kit. Expression levels of sodum\|potassium adenosine triphosphatase (NaK\|ATPase) and MCT1 were analyzed by Western blotting. Mitochondrial membrane potential was assessed by flow cytometry, and the phosphorylation status of MCT1 was determined by protein mass spectrometry. Immunofluorescent staining was performed to examine the co-localization of MCT1 with clathrin and caveolin-1 under low-glucose conditions or after treatment with the cAMP agonist forskolin. Finally, the effect of sodium pyruvate on MCT1 expression was evaluated. Results Low-glucose conditions triggered a significant stress response in OPCs. Under these conditions, phosphorylation of MCT1 enhanced its interaction with clathrin. Moreover, the translocation of MCT1 was dependent on cAMP signaling, and sodium pyruvate upregulated MCT1 expression. Conclusion Under low-glucose conditions, cAMP and clathrin facilitate the trafficking of phosphorylated MCT1 from the cytoplasm to the plasma membrane.

导出引用

张优利周鹏张晓艳. 低葡萄糖诱导少突胶质前体细胞中单核羧酸转运体1的胞质转运[J]. 解剖学报. 2026, 57(3): 306-312 https://doi.org/10.16098/j.issn.0529-1356.2026.03.005

ZHANG You-li, ZHOU Peng, ZHANG Xiao-yan. Cytoplasmic transport of monocarboxylate transporter 1 in oligodendrocyte precursor cells induced by low glucose[J]. Acta Anatomica Sinica. 2026, 57(3): 306-312 https://doi.org/10.16098/j.issn.0529-1356.2026.03.005

中图分类号： R322.8 R651.15

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