宝藿苷Ⅰ调节PINK1/Parkin信号通路对β淀粉样蛋白25~35诱导的SH-SY5Y细胞线粒体自噬和凋亡的影响

doi:10.16098/j.issn.0529-1356.2026.03.004

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解剖学报 ›› 2026, Vol. 57 ›› Issue (3) : 298-305. DOI: 10.16098/j.issn.0529-1356.2026.03.004

神经生物学

宝藿苷Ⅰ调节PINK1/Parkin信号通路对β淀粉样蛋白25~35诱导的SH-SY5Y细胞线粒体自噬和凋亡的影响

白艳梅¹ 杨国锋² 张真源² 高娟¹ 李静¹ 李恳¹ 张静¹崔妍莉¹ 王森^1*

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Effect of baohuosideⅠon Aβ_25-35-induced mitochondrial autophagy and apoptosis in SH-SY5Y cells by PINK1/Parkin signaling pathway

BAI Yan-mei¹, YANG Guo-feng², ZHANG Zhen-yuan², GAO Juan¹, LI Jing¹, LI Ken¹, ZHANG Jing¹, CUI Yan-li¹, WANG Sen¹*

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摘要

目的探讨宝藿苷Ⅰ（Bao Ⅰ）调节PTEN诱导激酶1/帕金蛋白（PINK1/Parkin）信号通路对β淀粉样蛋白25~35（Aβ_25~35）诱导的SH-SY5Y细胞线粒体自噬和凋亡的影响。方法对数生长期的SH-SY5Y细胞分为正常组、Aβ_25~35组、低浓度宝藿苷Ⅰ组(L-Bao Ⅰ)、高浓度宝藿苷Ⅰ组(H-Bao Ⅰ)、H-Bao Ⅰ+阴性对照（NC）小干扰RNA（siRNA）组、H-Bao Ⅰ+PINK1 siRNA组，细胞增殖、凋亡分别通过 MTT、流式细胞术检测；PINK1、Parkin mRNA表达水平以Real-time PCR检测；自噬水平、线粒体膜电位通过透射电子显微镜和JC-1检测；PINK1、Parkin、自噬相关蛋白Beclin-1、微管相关蛋白1轻链3 (LC3)Ⅱ、自噬底物蛋白P62、Bax、Bcl-2和LC3Ⅰ表达通过Western blotting检测。结果 Aβ_25~35组570 nm吸光度（A₅₇₀）、PINK1 mRNA、Parkin mRNA表达、线粒体膜电位、PINK1、Parkin、Beclin-1、Bcl-2和LC3Ⅱ/LC3Ⅰ表达低于正常组，凋亡率、P62和Bax表达高于正常组（P<0.05）；不同剂量的宝藿苷Ⅰ的A₅₇₀、PINK1 mRNA及Parkin mRNA表达、线粒体膜电位、PINK1、Parkin、Beclin-1、Bcl-2、LC3Ⅱ/LC3Ⅰ表达高于Aβ_25~35组，凋亡率、P62、Bax表达低于Aβ_25~35组（P<0.05）；H-Bao Ⅰ+ PINK1 siRNA组A570、PINK1 mRNA及Parkin mRNA表达、线粒体膜电位、PINK1、Parkin、Beclin-1、Bcl-2和LC3Ⅱ/LC3Ⅰ表达低于H-Bao Ⅰ+NC siRNA组，凋亡率、P62和Bax表达高于H-Bao Ⅰ+ NC siRNA组（P<0.05）。结论宝藿苷 Ⅰ可激活Aβ_25~35诱导的SH-SY5Y细胞线粒体自噬，抑制细胞凋亡，其机制与PINK1/Parkin信号通路有关。

Abstract

Objective To investigate the effect of baohuosideⅠ(BaoⅠ) on β-amyloid protein 25-35（Aβ_25-35）-induced mitochondrial autophagy and apoptosis in SH-SY5Y cells by adjusting the PTEN-induced kinase 1/Parkin (PINK1/Parkin) signaling pathway. Methods SH-SY5Y cells in logarithmic growth phase were separated into normal group, Aβ_25-35 group, low concentration baohuosideⅠ(L-Bao Ⅰ) group, high concentration baohuosideⅠ(H-Bao Ⅰ) group, H-Bao Ⅰ+ negative control（NC）small interfering RNA (siRNA）group, and H-Bao Ⅰ+PINK1 siRNA group. Each group was repeated three times. Cell proliferation and apoptosis were detected by MTT and flow cytometry, respectively. The mRNA expression levels of PINK1 and Parkin were detected by Real-time PCR. The autophagy level and mitochondrial membrane potential were detected by transmission electron microscopy and JC-1. The autophagy-related proteins Beclin 1, microtubule-associated protein 1 light chain 3(LC3)Ⅱ, autophagy substrate protein P62, Bax, rabbit anti-Bcl-2, and rabbit anti-LC3Ⅰ expression were detected by Western blotting. Results The Aβ_25-35 group had lower absorbance value at 570 nm (A₅₇₀), expression of PINK1 mRNA, Parkin mRNA, mitochondrial membrane potential, expression of PINK1, Parkin, autophagy related proteins Beclin-1, Bcl-2 and LC3Ⅱ/LC3Ⅰ than the normal group, and higher apoptosis rate, and expression of P62 and Bax than the normal group (P<0.05). The different dose BaoⅠgroups had higher A₅₇₀, expression of PINK1 mRNA, Parkin mRNA, mitochondrial membrane potential, expression of PINK1, Parkin, Beclin-1, Bcl-2 and LC3Ⅱ/LC3Ⅰthan the Aβ_{25-35} group, and lower apoptosis rate, and expression of P62 and Bax than the Aβ_25-35 group (P<0.05). The H-BaoⅠ+ PINK1 siRNA group had lower A₅₇₀, expression of PINK1 mRNA, Parkin mRNA, mitochondrial membrane potential, expression of PINK1, Parkin, Beclin-1, Bcl-2 and LC3Ⅱ/LC3Ⅰ than the H-Bao Ⅰ+ NC siRNA group, and higher apoptosis rate, and expression of P62 and Bax than the H-Bao Ⅰ+ NC siRNA group (P<0.05). Conclusion Bao Ⅰ can activate mitochondrial autophagy induced by Aβ_25-35 in SH-SY5Y cells, inhibit cell apoptosis, and its mechanism is related to the PINK1/Parkin signaling pathway.

导出引用

白艳梅杨国锋张真源高娟李静李恳张静崔妍莉王森. 宝藿苷Ⅰ调节PINK1/Parkin信号通路对β淀粉样蛋白25~35诱导的SH-SY5Y细胞线粒体自噬和凋亡的影响[J]. 解剖学报. 2026, 57(3): 298-305 https://doi.org/10.16098/j.issn.0529-1356.2026.03.004

BAI Yan-mei, YANG Guo-feng, ZHANG Zhen-yuan, GAO Juan, LI Jing, LI Ken, ZHANG Jing, CUI Yan-li, WANG Sen.

Effect of baohuosideⅠon Aβ_25-35-induced mitochondrial autophagy and apoptosis in SH-SY5Y cells by PINK1/Parkin signaling pathway

[J]. Acta Anatomica Sinica. 2026, 57(3): 298-305 https://doi.org/10.16098/j.issn.0529-1356.2026.03.004

中图分类号： R329.2 R285.5

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