目的 开发一种脑电图（EEG）/肌电图（EMG）与多脑区光纤记录联用的方法，以同步捕捉癫痫发作时大脑整体和特定脑区的神经元活动变化。 方法 以小鼠颅骨中缝为界，将头部分为左右两个区域，一侧植入EEG电极（EEG/EMG），另一侧在纹状体、海马区、内嗅皮层和丘脑埋植光纤，同步监测脑电、肌电和钙信号动态变化。 结果 通过EEG/EMG与多脑区光纤记录联用，观察小鼠不同行为状态下多脑区神经元活动、脑电以及肌电的差异；在海人藻酸（KA）诱导的癫痫模型中，小鼠大脑神经元异常同步化放电伴随纹状体、海马区、内嗅皮层和丘脑的钙信号变化，其中海马区的变化最早出现。 结论 成功实现小鼠EEG/EMG与多脑区光纤记录的联用，通过该方法在KA诱导的癫痫模型中同步记录了多脑区钙信号、脑电和肌电信号的异常变化。 

Objective To develop a method  of combining electroencephalogram(EEG) /electromyography (EMG) with multi-regional fiber photometry recording to simultaneously capture the changes of neuronal activity in the whole brain and specific brain regions during epileptic seizures.    Methods The mouse head was divided into left and right regions based on the middle suture of the skull. EEG electrodes (EEG/EMG) were implanted in one side, while optical fibers were implanted in the striatum, hippocampus, entorhinal cortex, and thalamus on the contralateral side to simultaneously monitor EEG, EMG, and calcium signal dynamics.    Results By combining EEG/EMG with multi-regional fiber photometry recording, differences in neuronal activity across brain regions, alongside EEG and EMG, were observed during different behavioral states. In a kainic acid (KA)-induced epilepsy model, abnormal synchronous neuronal discharges in the mouse brain were accompanied by calcium signal changes in the striatum, hippocampus, entorhinal cortex, and thalamus, with the earliest changes occurring in the hippocampus.    Conclusion The combined use of EEG/EMG and multi-brain-region fiber photometry is successfully implemented in mice. This method  synchronously recordes abnormal calcium signal changes across multiple brain regions, along with EEG and EMG, in the KA-induced epilepsy model.