神经周围网和小清蛋白阳性神经元在不同年龄段小鼠特定脑区分布的差异

doi:10.16098/j.issn.0529-1356.2025.05.002

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解剖学报 ›› 2025, Vol. 56 ›› Issue (5) : 515-523. DOI: 10.16098/j.issn.0529-1356.2025.05.002

神经生物学

神经周围网和小清蛋白阳性神经元在不同年龄段小鼠特定脑区分布的差异

伏媚^1,2 叶苇一² 侯恒浩² 陈迁^1,2*

作者信息 +

Differences of perineuronal nets and parvalbumin-positive neuron distribution across specific brain regions in young and aged mice

FU Mei^{1, 2} YE Wei-yi² HOU Heng-hao² CHEN Qian^{1, 2*}

Author information +

文章历史 +

摘要

目的探讨神经周围网（PNN）和小清蛋白（PV）阳性神经元在年轻和老年小鼠特定脑区分布的差异。方法年轻（45 d）小鼠和老年（350 d）小鼠每组4只，使用4%多聚甲醛进行灌注取脑，振动切片机进行切片，厚50 μm，紫藤凝集素（WFA）染色和小清蛋白抗体进行免疫荧光染色。针对小鼠前扣带回皮层（ACC）、桶状皮层第4层（S1BF L4）、纹状体（STR）以及海马CA2脑区进行荧光成像和定量分析，比较不同年龄组小鼠PNN和PV阳性神经元的数量及PNN对PV阳性神经元的包裹情况。结果与年轻小鼠相比，老年小鼠的ACC、S1BFL4以及纹状体中的PNN和PV阳性神经元的数量无显著变化，但老年小鼠中PNN对PV阳性神经元的包裹比例显著增加。在海马CA2脑区，PNN阳性神经元的数量显著增高，而PV阳性神经元的数量无明显变化。结论 PNN阳性神经元的数量以及PNN对PV阳性神经元的包裹程度在年轻小鼠和老年小鼠特定脑区差异具有显著性。

Abstract

Objective To investigate the differences of perineuronal net (PNN) and parvalbumin (PV)-positive neuron distribution across specific brain regions between young and aged mice. Methods Brains from young (45 days) and aged (350 days) mice (n=4 per group) were fixed with 4% paraformaldehyde, sectioned (50 μm) using a vibratome, and stained with Wisteria floribunda agglutinin (WFA) and PV immunofluorescence. Quantitative analyses of PNN-positive and PV-positive neurons, along with PNN encapsulation of PV-positive neurons, were performed in the anterior cingulate cortex (ACC), somatosensory cortex barrel field layer 4 (S1BF L4), striatum (STR), and hippocampal CA2 region. Results Aged mice exhibited no significant changes in PNN-positive or PV-positive neuron counts in ACC, S1BF L4, or STR compared to young mice, but showed significantly increased PNN encapsulation of PV-positive neurons. In hippocampal CA2, PNN-positive neurons increased significantly without PV-positive neuron alterations. Conclusion The differences in PNN-PV neuron interactions and PNN density exist in specific brain regions of young and aged mice.

导出引用

伏媚叶苇一侯恒浩陈迁. 神经周围网和小清蛋白阳性神经元在不同年龄段小鼠特定脑区分布的差异[J]. 解剖学报. 2025, 56(5): 515-523 https://doi.org/10.16098/j.issn.0529-1356.2025.05.002

FU Mei YE Wei-yi HOU Heng-hao CHEN Qian. Differences of perineuronal nets and parvalbumin-positive neuron distribution across specific brain regions in young and aged mice [J]. Acta Anatomica Sinica. 2025, 56(5): 515-523 https://doi.org/10.16098/j.issn.0529-1356.2025.05.002

中图分类号： R322.8

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