目的 体外构建可模拟在体肠道黏膜结构的三维仿生模型。方法  以具有绒毛隐窝结构的丝素蛋白为支架，Caco-2和 HT29-MTX-E12为种子细胞，与肌成纤维细胞进行体外三维共培养，构建肠黏膜上皮-肌成纤维细胞共培养模型，通过对细胞标志性蛋白及基因的检测探索模型细胞的生长、活性、组织学特性以及功能基因的表达。结果 1. 成功构建了肠黏膜上皮-肌成纤维细胞共培养模型，活性良好。2. 共培养模型中肠上皮细胞黏液分泌增加，muc2基因表达上调。结论 具有绒毛隐窝结构的肠黏膜上皮-肌成纤维细胞共培养能够促进肠上皮细胞功能性分化，能够更好地模拟在体肠道黏膜结构与功能。

Objective  To construct a three-dimensional bionic model of intestinal mucosal structure in vitro.   Methods Using fibroin protein with villous-crypt structure as scaffold and Caco-2 and HT29-MTX-E12 as seed cells, the intestinal mucosal epithelial-myofibroblast co-culture model was constructed by three-dimensional co-culture with myofibroblasts in vitro. The growth, activity, histological characteristics and expression of functional genes of model cells were investigated through the detection of cell signature proteins and genes.  Results  1. An intestinal mucosal epithelial-myofibroblast co-culture model was successfully constructed with good cell activity. 2. Mucous secretion increased and muc2 gene expression was up-regulated in the co-culture model.  Conclusion  The co-culture of intestinal mucosal epithelial-myofibroblasts with villous-crypt structure can promote the functional differentiation of intestinal epithelial cells and better simulate the structure and function of intestinal mucosa in vivo.