目的 探讨短期和长期高果糖饮食对小鼠海马脑区神经代谢物及焦虑与抑郁样行为的影响，以期揭示高果糖饮食在情绪障碍性疾病中的潜在机制，并为预防和治疗相关疾病提供实验依据。方法  实验选用C57BL/6J雄性小鼠，随机分为两组：对照组（标准饮食，n=10）和实验组（高果糖饮食，n=10）。每组小鼠分别在接受4周（短期）和8周（长期）饮食干预后进行体重及空腹血糖的检测，并通过氢质子磁共振波谱成像（1H-MRS）检测海马脑区代谢物水平，之后进行旷场实验、强迫游泳实验和悬尾实验评估小鼠的焦虑及抑郁样行为表现。结果  高果糖饮食4周诱发小鼠海马神经代谢物谷氨酸升高，而谷胱甘肽和肌醇降低，并伴随强迫游泳中的不动时间缩短。高果糖饮食8周导致体重和糖代谢异常，海马谷氨酸水平反转性降低，并诱发明显的焦虑样行为，而海马谷氨酸水平的降低与焦虑样行为的增强成显著负相关。结论  海马谷氨酸水平异常可能是长期高果糖饮食诱导焦虑样行为的关键因素之一。

 Objective  To investigate the effects of short-term and long-term high-fructose diets on hippocampal neurometabolites and anxiety and depression-like behaviors in mice, revealing the potential mechanisms of high-fructose diets in mood disorders and providing a experimental basis for the prevention and treatment of related diseases.  Methods C57BL/6J male mice were randomly divided into two groups, control group (standard diet, n=10) and experimental group (high-fructose diet, n=10). Four weeks (short-term) and eight weeks (long-term) later, each group of mice was examined for body weight and fasting blood glucose, and neurometabolites levels in the hippocampus were detected by hydrogen proton magnetic resonance spectroscopy(1H-MRS), followed by the open-field test, the forced-swimming test, and the tail-suspension test to evaluate anxiety-like and depression-like behaviors.   Results  High fructose diet for 4 weeks elevated glutamate levels and reduced glutathione and myo-inositol levels in mice, accompanied by shortened immobility time in the forced swim test. High fructose diet for 8 weeks not only led to abnormalities in body weight and glucose metabolism but also caused a reversal decrease in hippocampal glutamate levels and induced significant anxiety-like behaviors, and the decrease in hippocampal glutamate levels showed a significant negative correlation with the enhancement of anxiety-like behaviors.   Conclusion   Altered hippocampal glutamate levels may be a key contributing factor to the anxiety-like behaviors induced by long-term high-fructose diet.