宁夏汉族青年指长比与基质金属蛋白酶9基因3个位点多态性的关联性

杨梦怡; 张静; 牛世博; 党洁; 马占兵; 陆宏; 霍正浩; 徐彧; 沈丹

doi:10.16098/j.issn.0529-1356.2025.01.010

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解剖学报 ›› 2025, Vol. 56 ›› Issue (1) : 74-79. DOI: 10.16098/j.issn.0529-1356.2025.01.010

细胞和分子生物学

宁夏汉族青年指长比与基质金属蛋白酶9基因3个位点多态性的关联性

杨梦怡^1,2张静¹牛世博^1,2党洁^1,2马占兵^1,2陆宏^1,2*霍正浩^1,2*徐彧¹沈丹¹

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Association of digit ratio with polymorphisms at three loci of matrix metalloproteinase 9 gene in Ningxia Han youths

YANG Meng-yi^1,2 ZHANG Jing¹ NIU Shi-bo^1,2 DANG Jie^1,2 MA Zhan-bing^1,2 LU Hong^1,2 HUO Zheng-hao^1,2 XU Yu¹ SHEN Dan¹

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摘要

目的探讨指长比与基质金属蛋白酶9（MMP-9）基因rs17576、rs3918249、rs9509 3个位点单核苷酸多态性（SNP）的关联性。方法以804名宁夏汉族青年（男性399名，女性405名）为研究对象，采用数码相机拍摄手部正面照片，图像分析软件标记解剖点并测量双手各指指长（2D、3D、4D、5D）；多重PCR法对MMP-9基因3个多态性位点进行检测；SPSS 25.0及R Studio软件进行数据分析和绘图。结果宁夏汉族女性青年双手2D/3D（P<0.05）、2D/4D（左：P<0.01，右：P<0.05），右手2D/5D（P<0.01）、3D/5D、4D/5D（P<0.05）及左手3D/4D（P<0.05）均明显高于男性；MMP-9基因3个位点基因型和等位基因频率在性别间的差异均无统计学意义（P>0.05）；男性青年右手2D/4D与rs17576和rs3918249位点基因型显著关联（P<0.05）。结论MMP-9基因SNPs（rs17576和rs3918249）可能与宁夏汉族男性青年2D/4D的形成有关。

Abstract

Objective To investigate the association of digit ratio with single nucleotide polymorphism (SNP) at three loci (rs17576, rs3918249, rs9509) of matrix metallopeptidase 9 (MMP-9) gene. Methods A total of 804 Ningxia Han youths (399 males and 405 females) were used as the study subjects. A digital camera was used to take frontal photographs of the hands, and image analysis software was used to mark the anatomical points and measure the lengths of each finger of both hands (2D, 3D, 4D, 5D); Multiplexed PCR was used to detect the three polymorphic sites of the MMP-9 gene, SPSS 25.0 and R Studio software were used for data analysis and plotting. Results The 2D/3D (P<0.05) and 2D/4D (left, P<0.01, right, P<0.05) of both hands, 2D/5D (P<0.01), 3D/5D, 4D/5D (P<0.05) of the right hand, and 3D/4D (P<0.05) of the left hand in female youths of Ningxia Han were significantly higher than those in males, Differences in genotypes and allele frequencies at all 3 loci of the MMP-9 gene were not statistically significant between genders (P>0.05). Right hand 2D/4D was significantly associated with genotypes at the rs17576 and rs3918249 loci in male youths (P<0.05). Conclusion MMP-9 gene SNPs (rs17576 and rs3918249) may be associated with the formation of 2D/4D of Ningxia Han male youths.

关键词

基质金属蛋白酶9

/ 基因多态性 / 指长比 / 解剖学测量 / 多重聚合酶链反应 / 宁夏汉族青年

Key words

Matrix metallopeptidase 9

/ Genetic polymorphism / Digit ratio / Anatomical measurement / Multiplex PCR / Ningxia Han youth

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杨梦怡张静牛世博党洁马占兵陆宏霍正浩徐彧沈丹. 宁夏汉族青年指长比与基质金属蛋白酶9基因3个位点多态性的关联性[J]. 解剖学报. 2025, 56(1): 74-79 https://doi.org/10.16098/j.issn.0529-1356.2025.01.010

YANG Meng-yi ZHANG Jing NIU Shi-bo DANG Jie MA Zhan-bing LU Hong HUO Zheng-hao XU Yu SHEN Dan. Association of digit ratio with polymorphisms at three loci of matrix metalloproteinase 9 gene in Ningxia Han youths[J]. Acta Anatomica Sinica. 2025, 56(1): 74-79 https://doi.org/10.16098/j.issn.0529-1356.2025.01.010

中图分类号： Q983

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