河北医科大学人脑组织库样本分析

杜鹃; 米世雄; 靳玉川; 杨倩; 马敏; 赵雪汝; 刘风藏; 赵长义; 张展翅; 樊平; 崔慧先

doi:10.16098/j.issn.0529-1356.2024.04.009

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解剖学报 ›› 2024, Vol. 55 ›› Issue (4) : 437-444. DOI: 10.16098/j.issn.0529-1356.2024.04.009

脑科学研究论著

河北医科大学人脑组织库样本分析

杜鹃米世雄靳玉川杨倩马敏赵雪汝刘风藏赵长义张展翅樊平崔慧先^*

作者信息 +

Analysis of Human Brain Bank samples from Hebei Medical University

DU Juan MI Shi-xiong JIN Yu-chuan YANG Qian MA Min ZHAO Xue-ru LIU Feng-cang ZHAO Chang-yi ZHANG Zhan-chi FAN Ping CUI Hui-xian*

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摘要

目的通过分析河北医科大学人脑组织库样本基本情况，了解河北省人脑组织捐献现状，为后续科学研究提供基本数据支撑。方法对河北医科大学人脑组织库收集样本进行统计(2019年12月~2024年2月), 分析性别、年龄、死亡原因等基本资料，以及死亡后取材时间、脑脊液pH值、RNA完整性等质量控制信息和神经病理学诊断结果等内容。结果截止到2024年2月，河北医科大学人脑组织库共收集保存人脑样本30例, 男女比例9∶1，其中80岁以上者占53%，死亡原因为心脑血管疾病36.67% 与神经系统疾病23.33% 占比较高。脑组织捐献者归属地在石家庄市内的占90%，市外的占10%。死亡后取材延误时间较短，12 h以内的占90%，大于12 h占10%。RNA完整性（RIN）值 > 6的脑样本占69.23%。脑脊液pH 5.8~7.5，平均值6.60 ± 0.45。成年人脑重906~1496 g，平均值（1210.78 ± 197.84）g。检出3种载脂蛋白E (APOE)亚型、5种APOE基因型（ε2/ε3、ε2/ε4、ε3/ε3、ε3/ε4、ε4/ε4）。涉及神经病理学诊断相关染色11种，均已建立起系统化染色流程并使用。神经病理学诊断为神经退行性疾病样本共计12例（包括阿尔茨海默病、帕金森病、多系统萎缩、皮质基底节变性和进行性核上性麻痹等），占比40%，80岁以上脑样本共病率达100%。结论河北医科大学脑库脑组织捐献者的资料总结与统计学分析，可以反映河北省人脑组织库建设与运行的现状，也可以更有针对性地了解并识别可能捐献的群体，为脑库的建设提供参考，为科学研究使用提供更为可靠的材料和数据支持。

Abstract

Objective To understand the current situation of human brain donation in Hebei Province by analyzing the basic information of Human Brain Bank samples of Hebei Medical University in order to provide basic data support for subsequent scientific research. Methods The samples collected from the Human Brain Bank of Hebei Medical University were analyzed (from December 2019 to February 2024), including gender, age, cause of death, as well as quality control data such as postmortem delay time, pH value of cerebrospinal fluid and and RNA integrity number and result of neuropathological diagnosis. Results Until February 2024, 30 human brain samples were collected and stored in the Human Brain Bank of Hebei Medical University, with a male to female ratio of 9∶1. Donors over 80 years old accounted for 53%. Cardiovascular and cerebrovascular diseases (36.67%) and nervous system diseases (23.33%) accounted for a high proportion of the death causes. The location of brain tissue donors in Shijiazhuang accounted for 90% donations, and the others were from outside the city. The postmortem delay time was relatively short, 90% within 12 hours and 10 % more than 12 hours. 69.23% of the brain samples had RNA integrity values greater than 6. Cerebrospinal fluid pH values ranged from 5.8 to 7.5, with an average value of 6.60 ± 0.45. Brain weights ranged from 906-1496g, with an average value of（1210.78 ± 197.84）g. Three apolipoprotein E (APOE) alleles were detected including five genotypes (ε2/ε3, ε2/ε4, ε3/ε3, ε3/ε4, ε4/ε4). Eleven staining methods related to neuropathological diagnosis had been established and used. A total of 12 cases were diagnosed as neurodegenerative diseases (including Alzheimer’s disease, Parkinson’s disease, multiple system atrophy, corticobasal degeneration and progressive supranuclear palsy，etc.), accounting for 40% donated brains. The comorbidity rate of samples over 80 years old was 100%. Conclusion The summary and analyses of the data of brain donors in the Human Brain Bank of Hebei Medical University can reflect the current situation of the construction and operation of the brain bank in Hebei Province, and it can also be more targeted to understand and identify potential donors. Our information can provide reference for the construction of brain bank and provides more reliable materials and data support for scientific research.

关键词

人脑组织库

/ 人脑样本 / 基本资料 / 质量控制信息 / 神经病理学诊断 / 神经退行性疾病 / 共病

Key words

Human Brain Bank / Human brain sample / Basic information / Quality control information / Neuropathological diagnosis / Neurodegenerative disease / Comorbidity

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杜鹃米世雄靳玉川杨倩马敏赵雪汝刘风藏赵长义张展翅樊平崔慧先. 河北医科大学人脑组织库样本分析[J]. 解剖学报. 2024, 55(4): 437-444 https://doi.org/10.16098/j.issn.0529-1356.2024.04.009

DU Juan MI Shi-xiong JIN Yu-chuan YANG Qian MA Min ZHAO Xue-ru LIU Feng-cang ZHAO Chang-yi ZHANG Zhan-chi FAN Ping CUI Hui-xian. Analysis of Human Brain Bank samples from Hebei Medical University[J]. Acta Anatomica Sinica. 2024, 55(4): 437-444 https://doi.org/10.16098/j.issn.0529-1356.2024.04.009

中图分类号： R741

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