新生小鼠大脑中生长激素受体阳性神经元的分布和功能

夏宇; 任淑裕; 李涛; 梅峰

doi:10.16098/j.issn.0529-1356.2024.04.007

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解剖学报 ›› 2024, Vol. 55 ›› Issue (4) : 422-429. DOI: 10.16098/j.issn.0529-1356.2024.04.007

脑科学研究论著

新生小鼠大脑中生长激素受体阳性神经元的分布和功能

夏宇任淑裕李涛梅峰^*

作者信息 +

Function and distribution of growth hormone receptor positive neurons in neonatal mouse brain

XIA Yu REN Shu-yu LI Tao MEI Feng^*

Author information +

文章历史 +

摘要

目的探讨生长激素受体(GHR)阳性神经元在新生小鼠大脑中的分布及功能。方法选取6只GHR-CreERT; mTOMATO/mGFP荧光报告基因小鼠，均在出生后第3天使用他莫昔芬诱导，在第10天和第17天分别取材3只，观察不同发育阶段大脑中GHR阳性神经细胞的分布。构建GHR floxed小鼠，通过与神经元特异性Thy1-CreERT; YFP小鼠杂交，诱导性敲除神经元中GHR。对照小鼠（GHR fl/fl 4只）和条件敲除小鼠（Thy1; GHR fl/fl 4只），在出生后第3天诱导，于第10天取材，用于观察神经元GHR信号对大脑发育的影响。结果 GHR荧光报告基因小鼠显示GHR阳性细胞在新生小鼠大脑中广泛表达，GHR阳性神经元集中在下丘脑室旁核(PVN)；特异性敲除新生小鼠大脑神经元中的GHR，神经元以及各类胶质细胞的单位面积数量未见明显差异。结论 GHR阳性神经元主要集中在下丘脑PVN，敲除发育大脑神经元的GHR不能明显改变各种神经元和神经胶质等细胞的形态和密度。

Abstract

Objective To study the distribution and effect of growth hormone receptor (GHR) positive neurons in neonatal mouse brain Methods Six GHR-CreERT;mTOMATO/mGFP fluorescent reporter gene mice were selected. All of them were induced with tamoxifen on the 3rd day after birth. On the 10th and 17th days, 3 mice were sampled respectively at each time point. The distribution of GHR-positive neurons in the developing brain was observed at different stages. GHR floxed mice were generated. By crossing them with neuron-specific Thy1-CreERT;YFP mice, GHR was induced to be knocked out in neurons. Control mice (GHR fl/fl, 4 mice) and conditional knockout mice (Thy1-CreERT;YFP;GHR fl/fl, 4 mice) were induced on the 3rd day after birth. Samples were collected on the 10th day for observing the effect of neuronal GHR signaling on brain development. Results The GHR fluorescent reporter gene mice showed that GHR-positive cells are widely expressed in the developing mouse brain. GHR-positive neurons are concentrated in the paraventricular hypothalamic nucleus (PVN). After specifically knocking out GHR in neurons of the developing mouse brain, no significant differences were observed in the areal densities of neurons and various types of glial cells. Conclusion GHR-positive neurons are mainly concentrated in the PVN. Knocking out GHR in neurons of the developing brain cannot significantly change the morphology and density of various neural cell types.

导出引用

夏宇任淑裕李涛梅峰. 新生小鼠大脑中生长激素受体阳性神经元的分布和功能[J]. 解剖学报. 2024, 55(4): 422-429 https://doi.org/10.16098/j.issn.0529-1356.2024.04.007

XIA Yu REN Shu-yu LI Tao MEI Feng. Function and distribution of growth hormone receptor positive neurons in neonatal mouse brain[J]. Acta Anatomica Sinica. 2024, 55(4): 422-429 https://doi.org/10.16098/j.issn.0529-1356.2024.04.007

中图分类号： R329.4

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