小鼠中缝背核向屏状核投射神经通路的形态学特征

巩涛; 蔡志平; 史娟; 李云庆

doi:10.16098/j.issn.0529-1356.2024.04.006

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解剖学报 ›› 2024, Vol. 55 ›› Issue (4) : 414-421. DOI: 10.16098/j.issn.0529-1356.2024.04.006

脑科学研究论著

小鼠中缝背核向屏状核投射神经通路的形态学特征

巩涛^1,2蔡志平¹史娟^2*李云庆^2*

作者信息 +

Morphological features of the neural projection pathway from the dorsal raphe nucleus to the claustrum in mice

GONG Tao ^1,2CAI Zhi-ping¹SHI Juan^2* LI Yun-qing^1,2*

Author information +

文章历史 +

摘要

目的探索小鼠中缝背核（DRN）向屏状核（CLA）投射神经通路的形态学特征。方法分别将逆行示踪剂594 retrobeads和顺行示踪剂生物素化的葡聚糖胺（BDA）注入CLA（n=3）和DRN（n=3），结合5-羟色胺（5-HT）免疫荧光染色，观察逆行标记神经元的分布和神经化学特征以及顺标神经纤维和终末在全脑和CLA内的分布；利用钙离子/钙调蛋白依赖性蛋白激酶Ⅱ（CaMKⅡ)-Cre、谷氨酸脱羧酶67（GAD67）-Cre动物结合狂犬逆行跨突触病毒（RV）标记，观察DRN-CLA通路的下游靶神经元类型。结果 DRN的吻、中、尾段均能观察到594 retrobeads阳性神经元的胞体，中段的腹侧亚核（DRV）分布较多，且90%以上的逆行标记神经元为5-HT阳性。BDA顺标纤维密集分布于腹侧被盖区、束旁核、腹侧苍白球、杏仁中央核等区，CLA内的神经纤维和终末相对稀疏。RV跨突触逆行标记结果显示，CaMKⅡ-Cre动物的DRN中含有较多的突触前神经元，而GAD67-Cre动物DRN中突触前逆行标记神经元较少。结论 DRN-CLA通路是以DRV分布为主的5-HT能投射通路，其在CLA内的纤维和终末较为稀疏，主要与CLA内的CaMKⅡ阳性神经元形成突触联系。

Abstract

Objective To explore the morphological features of the neural projection pathway from the dorsal raphe nucleus (DRN) to the claustrum (CLA) in mice. Methods After injection of the retrograde tracer 594 retrobeads into the right CLA (n=3) or the anterograde tracer biotinylated dextran amine(BDA) into the DRN (n=3), the distribution and the neurochemical feature of the retrogradely labeled neurons in the DRN, as well as the locations of nerve fibers and terminals throughout the brain and within the CLA, were observed by a combined immunofluorescent staining for 5-hydrozytryptamine(5-HT). The downstream target of the DRN-CLA projection was studied by injection of rabies retrograde monosynaptic virus (RV) into calcium ion/calmodulin dependent protein kinaeⅡ(CaMKⅡ)-Cre or glutamate decarboxylase 67(GAD67)-Cre mice. Results Retrograde labeled neurons were observed in the rostral, middle, and caudal segments of the DRN, with those in the middle segment and in the ventral part of the nucleus (DRV) the densest. Over 90% of the retrograde labeled neurons were 5-HT positive. After injection of BDA into the DRN, dense fiber projections were observed in the ventral tegmental area, parafascicular nucleus, ventral pallidum, and central amygdala nucleus. In comparison, DRN derived fibers and terminals in the CLA were relatively sparse. After injection of RV trans-synaptic virus into the CLA of CaMKⅡ-Cre and GAD67-Cre mice, respectively, dense RV-labeled presynaptic neurons were observed in the DRN of CaMKⅡ-Cre mice, while those in the DRN of GAD67-Cre mice were rather sparse.Conclusion The DRN-CLA projection pathway is characterized by the dense 5-HTergic neurons in the DRV, the relatively sparse fibers or terminals in the CLA, and a primary synaptic connection with CaMKⅡ positive neurons in the CLA.

导出引用

巩涛蔡志平史娟李云庆. 小鼠中缝背核向屏状核投射神经通路的形态学特征[J]. 解剖学报. 2024, 55(4): 414-421 https://doi.org/10.16098/j.issn.0529-1356.2024.04.006

GONG Tao CAI Zhi-ping SHI Juan LI Yun-qing. Morphological features of the neural projection pathway from the dorsal raphe nucleus to the claustrum in mice[J]. Acta Anatomica Sinica. 2024, 55(4): 414-421 https://doi.org/10.16098/j.issn.0529-1356.2024.04.006

中图分类号： R322

参考文献

［1］Fu W, Le Maître E, Fabre V, et al. Chemical neuroanatomy of the dorsal raphe nucleus and adjacent structures of the mouse brain［J］. J Comp Neurol, 2010, 518(17): 3464-3494.

［2］Chen YB, Bai Y, Huang J, et al. Serotoninergic projection from dorsal raphe nucleus to insular cortex is involved in acute itch sensation processing in mice［J］. Brain Res, 2019, 1715: 224-234.

［3］Li L, Zhang LZ, He ZX, et al. Dorsal raphe nucleus to anterior cingulate cortex 5-HTergic neural circuit modulates consolation and sociability［J］. Elife, 2021, 10: e67638.

［4］Zhou W, Jin Y, Meng Q, et al. A neural circuit for comorbid depressive symptoms in chronic pain［J］. Nat Neurosci, 2019, 22(10): 1649-1658.

［5］Bruguier H, Suarez R, Manger P, et al. In search of common developmental and evolutionary origin of the claustrum and subplate［J］. J Comp Neurol, 2020, 528(17): 2956-2977.

［6］Marriott BA, Do AD, Zahacy R, et al. Topographic gradients define the projection patterns of the claustrum core and shell in mice［J］. J Comp Neurol, 2021, 529(7): 1607-1627.

［7］Remedios R, Logothetis NK, Kayser C. Unimodal responses prevail within the multisensory claustrum［J］. J Neurosci, 2010, 30(39): 12902-12907.

［8］Smith JB, Lee AK, Jackson J. The claustrum［J］. Curr Biol, 2020, 30(23): R1401-R1406.

［9］Biemond A. The conduction of pain above the level of the thalamus opticus［J］. AMA Arch Neurol Psychiatry, 1956, 75(3): 231-244

［10］Koubeissi MZ, Bartolomei F, Beltagy A, et al. Electrical stimulation of a small brain area reversibly disrupts consciousness［J］. Epilepsy Behav, 2014, 37: 32-35.

［11］Goll Y, Atlan G, Citri A. Attention: the claustrum［J］. Trends Neurosci, 2015, 38(8): 486-495.

［12］Billwiller F, Renouard L, Clement O, et al. Differential origin of the activation of dorsal and ventral dentate gyrus granule cells during paradoxical (REM) sleep in the rat［J］. Brain Struct Funct, 2017, 222(3): 1495-1507.

［13］Zingg B, Dong HW, Tao HW, et al. Input-output organization of the mouse claustrum［J］. J Comp Neurol, 2018, 526(15): 2428-2443.

［14］Smith JB, Alloway KD, Hof PR, et al. The relationship between the claustrum and endopiriform nucleus: a perspective towards consensus on cross-species homology［J］. J Comp Neurol, 2019, 527(2): 476-499.

［15］Torgerson CM, Irimia A, Goh SY, et al. The DTI connectivity of the human claustrum［J］. Hum Brain Mapp, 2015, 36(3): 827-838.

［16］Fernández-Miranda JC, Rhoton AL Jr, Kakizawa Y, et al. The claustrum and its projection system in the human brain: a microsurgical and tractographic anatomical study［J］. J Neurosurg, 2008, 108(4): 764-774.

［17］Wang Q, Wang Y, Kuo HC, et al. Regional and cell-type-specific afferent and efferent projections of the mouse claustrum［J］. Cell Rep, 2023, 42(2): 112-118.

［18］Huang KW, Ochandarena NE, Philson AC, et al. Molecular and anatomical organization of the dorsal raphe nucleus［J］. Elife, 2019, 8: e46464.

［19］Muzerelle A, Scotto-Lomassese S, Bernard JF, et al. Conditional anterograde tracing reveals distinct targeting of individual serotonin cell groups (B5-B9) to the forebrain and brainstem［J］. Brain Struct Funct, 2016, 221(1): 535-561.

［20］Barrett FS, Krimmel SR, Griffiths RR, et al. Psilocybin acutely alters the functional connectivity of the claustrum with brain networks that support perception, memory, and attention［J］. Neuroimage, 2020, 218: 116980.

［21］Palacios JM. Serotonin receptors in brain revisited［J］. Brain Res, 2016, 1645: 46-49.

［22］Koyama Y, Kondo M, Shimada S. Building a 5-HT3A receptor expression map in the mouse brain［J］. Sci Rep, 2017,7: 42884.

［23］Norimoto H, Fenk LA, Li HH, et al. A claustrum in reptiles and its role in slow-wave sleep［J］. Nature, 2020, 578(7795): 413-418.

［24］Xu QY, Zhang HL, Du H, et al. Identification of a glutamatergic claustrum-anterior cingulate cortex circuit for visceral pain processing［J］. J Neurosci, 2022, 42(43): 8154-8168.

［25］Biemond A. The conduction of pain above the level of the thalamus opticus［J］. AMA Arch Neurol Psychiatry, 1956, 75(3): 231-244.

［26］Oikonomou G, Altermatt M, Zhang RW, et al. The serotonergic raphe promote sleep in zebrafish and mice［J］. Neuron, 2019, 103(4): 686-701.

［27］Wu YY, Jiang YL, Shao XM, et al. Comparisons of 5-hydroxytryptamine expression at different levels of dorsal raphe nucleus in rats with pain-depression dyad［J］. Acta Anatomica Sinica, 2015, 46(2): 170-174. (in Chinese)

吴媛媛, 蒋永亮, 邵晓梅，等.痛抑郁二联征模型大鼠中缝背核不同水平5羟色胺表达差异［J］. 解剖学报, 2015, 46(2): 170-174.

［28］Bardin L. The complex role of serotonin and 5-HT receptors in chronic pain［J］. Behav Pharmacol, 2011, 22(5-6): 390-404.