中年男性精子DNA碎片率对体外受精-胚胎移植妊娠结局的影响

王平; 王静; 陈先兵; 陈曦

doi:10.16098/j.issn.0529-1356.2024.03.013

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解剖学报 ›› 2024, Vol. 55 ›› Issue (3) : 345-348. DOI: 10.16098/j.issn.0529-1356.2024.03.013

组织学胚胎学发育生物学

中年男性精子DNA碎片率对体外受精-胚胎移植妊娠结局的影响

王平王静陈先兵陈曦*

作者信息 +

Effect of sperm DNA fragmentation on in vitro fertilization-embryo transfer outcome among middle-aged men

WANG Ping WANG Jing CHEN Xian-bing CHEN Xi*

Author information +

文章历史 +

摘要

目的探讨中年男性精子DNA碎片率（DFI）与精液质量和体外受精胚胎移植妊娠结局的关系。方法共收集180例接受常规体外受精胚胎移植治疗且男性年龄>38岁的精液标本。根据DFI的阈值分成（<30%和≥30%）两组。主要测量指标包括：常规精液参数、激素水平、DFI、受精率、优质胚胎率、临床妊娠率等。结果通过比较分析发现，DFI与男性卵泡刺激素(FSH)、精子活力、精子形态密切相关，且精子活力随着DFI水平的升高而下降（P<0.05）；当DFI≥30%时，优质胚胎率下降（P<0.05），但两组的临床妊娠率差异无显著性（P>0.05）。结论 DFI可以作为中年男性精液常规分析的重要参考指标，虽然DFI影响优质胚胎率，但与辅助生殖治疗的临床妊娠结局无关。

Abstract

Objective To explore the relationship between sperm DNA fragmentation index(DFI) and sperm quality, as well as in vitro fertilization-embryo transfer (IVF-ET) preganancy outcomes in middle-aged men. Methods A total of 180 semen specimens from males aged over 38 years who received comventional IVF-ET treatment were collected and divided into two groups(<30% and ≥30%) based on the DIF threshold. Main measures result including: normal semen parameters, hormone levels, DFI, fertilization rate, good quality embryo rate and pregnancy rate. Results DFI was closely related to male follicle-stimulating hormone（FSH）, sperm motility，morphology, and the sperm vitality decreased with increasing DFI level (P<0.05). When DFI≥30%, the rate of high-quality embryos decreased (P<0.05), but there was no significant difference in the pregnancy rate (P>0.05). Conclusion DFI can serve as an important indicator for routine analysis of semen in middle-aged men. DFI affects the rate of high-quality embryos, but it is not related to the clinical pregnancy outcome of assistant reproduction technology (ART).

导出引用

王平王静陈先兵陈曦. 中年男性精子DNA碎片率对体外受精-胚胎移植妊娠结局的影响[J]. 解剖学报. 2024, 55(3): 345-348 https://doi.org/10.16098/j.issn.0529-1356.2024.03.013

WANG Ping WANG Jing CHEN Xian-bing CHEN Xi. Effect of sperm DNA fragmentation on in vitro fertilization-embryo transfer outcome among middle-aged men[J]. Acta Anatomica Sinica. 2024, 55(3): 345-348 https://doi.org/10.16098/j.issn.0529-1356.2024.03.013

中图分类号： R714.8

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