微小RNA-30a调控丝裂原活化蛋白激酶通路对主动脉夹层大鼠的影响

吴跃武; 胡斌; 过小冬

doi:10.16098/j.issn.0529-1356.2024.02.014

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解剖学报 ›› 2024, Vol. 55 ›› Issue (2) : 222-228. DOI: 10.16098/j.issn.0529-1356.2024.02.014

组织学胚胎学发育生物学

微小RNA-30a调控丝裂原活化蛋白激酶通路对主动脉夹层大鼠的影响

吴跃武¹ 胡斌^2* 过小冬¹ 付琴¹ 邹志佳¹

作者信息 +

Effect of microRNA-30a regulation of mitogen-activated protein kinase pathway on aortic coarctation in rats

WU Yue-wu¹ HU Bin^2* GUO Xiao-dong¹ FU Qin¹ ZOU Zhi-jia¹

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文章历史 +

摘要

目的探讨微小RNA (miR)-30a调控MAPK通路对主动脉夹层大鼠模型夹层形成、炎性因子及血管收缩的影响。方法选取SD大鼠50只，建立主动脉夹层大鼠模型，将其随机分为对照组、模型组、miR-NC组、miR-30a组、miR-30a抑制剂组，各组10只。组织病理学染色观察大鼠主动脉组织变化、主动脉中膜弹力纤维与胶原纤维变化；采用PCR、尾动脉压力计、ELISA法对各组miR-30a表达、干预前后收缩压情况、血清炎性因子表达进行检测；采用Western blotting检测各组大鼠基质金属蛋白酶（MMP）-6、MMP-2蛋白表达以及MAPK通路相关蛋白表达。结果 MiR-30a抑制剂组血管壁撕裂程度和内动脉壁排列紊乱有所改善；miR-30a抑制剂组改善血管重构；与对照组相比，模型组miR-30a表达较高，与miR-NC组相比，miR-30a组、miR-30a抑制剂组表达较低，P<0.05；干预前，各组收缩压比较差异无统计学意义，P>0.05；与对照组相比，模型组收缩压较高，与miR-NC组相比，miR-30a组表达较高，miR-30a抑制剂组表达较低，P<0.05；与对照组相比，模型组肿瘤坏死因子（TNF)、白细胞介素(IL)-6、IL-1β表达较高，与miR-NC组相比，miR-30a组表达较高，miR-30a抑制剂组表达较低，P<0.05；与对照组相比，模型组、MMP-6、MMP-2、Ras、Raf、P38 MAPK及ERK1/2蛋白表达较高，与miR-NC组相比，miR-30a组表达较高，miR-30a抑制剂组表达较低，P<0.05。结论 MiR-30a参与主动脉夹层的形成、炎症反应、调控主动脉夹层血管重构，可能是通过调控MAPK信号通路实现的。

Abstract

Objective To investigate the effects of microRNA (miR)-30a-regulated MAPK pathway on the formation of intercalation, inflammatory factors and vasoconstriction in a rat model of aortic coarctation. Methods Fifty SD rats were selected to establish the rat model of aortic coarctation, and were randomly divided into control group, model group, miR-NC group, miR-30a group and miR-30a inhibitor group, 10 rats in each group. Histopathological changes in the aortic tissue and changes in the elastic fibers and collagen fibers of the aortic mesothelium were observed; The expression of miR-30a, systolic blood pressure before and after the intervention and the expression of serum inflammatory factors in each group were measured by PCR, tail artery manometry and ELISA; Matrix metalloproteinase (MMP)-6, MMP-2 protein expression and MAPK pathway were measured by Western blotting in each group. The expression of MMP-6, MMP-2 and MAPK pathway related proteins were measured by Western blotting. Results The miR-30a inhibitor group improved the degree of vessel wall tearing and disorganized internal arterial wall arrangement; The miR-30a group improved vascular remodeling; miR-30a expression was higher in the model group compared with the control group, and lower in the miR-30a group and miR-30a inhibitor group compared with the miR-NC group, P<0.05; Before the intervention, the difference in systolic blood pressure between the groups compared was not statistically significant, P> 0.05; Compared with the control group, systolic blood pressure was higher in the model group, higher expression in the miR-30a group and lower expression in the miR-30a inhibitor group compared with the miR-NC group, P< 0.05; compared with the control group, tumor necrosis factor(TNF)-α, interleukin (IL)-6, IL-1β expression was higher in the model group, higher expression in the miR-30a group compared with the miR-NC group, lower expression in the miR-30a inhibitor group, P< 0.05; higher expression of TNF-α, MMP-6, MMP-2, Ras, Raf, P38MAPK, ERK1/2 proteins in the model group compared with the control group, higher expression in the miR-30a group compared with the miR-NC group, lower expression in the miR-30a inhibitor group, P<0.05. Conclusion MiR-30a is involved in the process of aortic coarctation formation, inflammatory response, and regulation of aortic coarctation vascular remodeling, possibly through regulation of the MAPK signaling pathway.

导出引用

吴跃武胡斌过小冬付琴邹志佳. 微小RNA-30a调控丝裂原活化蛋白激酶通路对主动脉夹层大鼠的影响[J]. 解剖学报. 2024, 55(2): 222-228 https://doi.org/10.16098/j.issn.0529-1356.2024.02.014

WU Yue-wu HU Bin GUO Xiao-dong FU Qin ZOU Zhi-jia. Effect of microRNA-30a regulation of mitogen-activated protein kinase pathway on aortic coarctation in rats[J]. Acta Anatomica Sinica. 2024, 55(2): 222-228 https://doi.org/10.16098/j.issn.0529-1356.2024.02.014

中图分类号： R654.3

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