目的 探讨慢病毒载体介导的类固醇生成因子-1（SF-1）沉默对人子宫内膜基质细胞（hESCs）蜕膜化进程的影响及机制。 方法 收集子宫内膜异位症（EM）患者与正常妊娠者的子宫内膜组织，通过Real-time PCR和免疫组织化学染色检测SF-1的差异表达情况。从正常妊娠者的子宫内膜组织中分离并鉴定hESCs，将hESCs分为对照组、雌二醇（E₂+孕酮（P4）组、短发夹RNA（shRNA,sh）-正常对照（NC+E₂+P4组、sh-SF-1+E₂+P4组，进行对应处理后，倒置显微镜下观察hESCs形态变化，Real-time PCR和Western blotting分别检测细胞内人胰岛素样生长因子结合蛋白1（IGFBP-1）、泌乳素（PRL）的mRNA表达水平和蛋白表达水平，流式细胞术测定细胞周期分布，免疫荧光染色观察细胞内自噬标记物微管相关蛋白轻链3（LC3）表达情况，Western blotting测定细胞内自噬相关蛋白LC3-Ⅱ、LC3-Ⅰ、Beclin-1表达水平。  结果 EM患者子宫内膜组织中SF-1 mRNA相对表达量和SF-1蛋白阳性率均高于正常妊娠者子宫内膜组织（P<0.05）。与sh-NC+E₂+P4组比较，sh-SF-1+E₂+P4组细胞多为长梭形，未见明显蜕膜化，IGFBP1、PRL的 mRNA相对表达量与蛋白相对表达量均显著下调（P<0.05），G₀/G₁期细胞比例显著减少而S期细胞比例显著增加（P<0.05），细胞内LC3荧光表达增强，LC3-Ⅱ/LC3-Ⅰ比值显著升高，Beclin-1蛋白相对表达量也显著上调（P<0.05）。  结论 EM 患者子宫内膜组织内SF-1表达升高，通过慢病毒载体介导的SF-1沉默能够抑制hESCs蜕膜化过程，该作用可能与调控细胞自噬水平相关。

Objective To explore the influence and mechanism of lentiviral vector-mediated steroidogenic factor-1 (SF-1) silence on decidual process of human endometrial stromal cells (hESCs).   Methods The endometrial tissues of patients with endometriosis (EM) and normal pregnant women were collected, and the differential expression of SF-1 was detected by Real-time PCR and immunohistochemical staining. hESCs were isolated from the endometrial tissue of normal pregnancy and identified, hESCs were divided into control group, estradiol (E₂) + progesterone (P4) group, short hairpin RAN(shRNA, sh)-normal control(NC)+E₂+P4 group, sh-SF-1+E₂+P4 group, after the corresponding processing, the morphological changes of hESCs were observed under an inverted microscope. Real-time PCR and Western blotting were used to detect the mRNA expression levels and protein levels of human insulin-like growth factor-binding protein-1 (IGFBP-1) and prolactin (PRL) in cells, respectively, flow cytometry was used to determine the cycle distribution of the cells, immunofluorescence staining was used to observe the expression of the intracellular auto-bid microtubule-associated protein light chain 3(LC3), Western blotting was used to determine the protein level of intracellular autophagy-related proteins LC3-Ⅱ, LC3-Ⅰ and Beclin-1.   Results The relative expression of SF-1 mRNA and the positive rate of SF-1 protein in endometrial tissue of EM patients were higher than those of normal pregnancy endometrial tissue (P<0.05). Compared with the sh-NC+E₂+P4 group, the cells in the sh-SF-1+E₂+P4 group were mostly long-spindle, and there was no obvious decidualization. The relative mRNA expression and protein expression of IGFBP1 and PRL were significantly down-regulated (P<0.05), the proportion of cells in G₀/G₁ phase was significantly decreased, the proportion of cells in S phase was significantly increased (P<0.05), the ratio of LC3-Ⅱ/LC3-Ⅰ was significantly increased, and the relative expression of Beclin-1 protein was also significantly up-regulated (P<0.05).   Conclusion The expression of SF-1 in the endometrial tissue of EM patients is increased, and SF-1 silencing mediated by lentiviral vector can inhibit the decidualization process of hESCs, which may be related to regulating the level of autophagy.