PDF(786 KB)
宁夏大学生示指和环指指长比与同源盒A11基因位点多态性的关联性
杨梦怡 牛世博 张静 党洁 马占兵 陆宏 霍正浩
解剖学报 ›› 2024, Vol. 55 ›› Issue (1) : 62-66.
PDF(786 KB)
PDF(786 KB)
宁夏大学生示指和环指指长比与同源盒A11基因位点多态性的关联性
杨梦怡 牛世博 张静 党洁 马占兵 陆宏* 霍正浩*
Association between index finger and ring finger length ratios and polymorphism of homeobox A11 gene locus among Ningxia college students
YANG Meng-yi NIU Shi-bo ZHANG Jing DANG Jie MA Zhan-bing LU Hong* HUO Zheng-hao*
目的 探讨宁夏大学生示指和环指指长比（2D∶4D）与同源盒（HOX）A11基因4个位点（rs6461992、rs6968828、rs7801581、rs17427875）多态性的关联性。 方法 采用数码相机提取667名宁夏汉族大学生（男性348名，女性319名）手部正面照片，图像分析软件标记解剖点并测量双手示指及环指指长；多重PCR法检测HOXA11基因各位点多态性；统计学软件比较分析2D∶4D和基因多态性在不同性别间的差异及其关联。 结果 宁夏汉族大学生女性左手和右手2D∶4D均显著高于男性（均P<0.05），右手-左手2D∶4D性别差异不显著；HOXA11基因rs7801581位点基因型、等位基因频率在不同性别间差异显著（均P<0.05），其他位点多态性在性别间的差异均无统计学意义；2D∶4D与HOXA11基因位点多态性关系中，仅女性左手 2D∶4D与 rs6461992位点基因型显著关联（P<0.05）。 结论 宁夏汉族大学生2D∶4D性别间差异显著，HOXA11基因rs6461992位点多态性可能与女性 2D∶4D 的形成有关。
Objective To investigate the association between the index finger and ring finger length ratio (2D∶4D) and of four loci (rs6461992, rs6968828, rs7801581, rs17427875) polymorphism of homeobox (HOX) A11 gene among Ningxia college students. Methods Digit camera was used to collect frontal hand photos of 667 Han college students (348 males and 319 females) from Ningxia province; Image analysis software was used to mark the anatomical points and measure finger lengths of the index and ring fingers of both hands; multiplex PCR was used to detect each locus polymorphisms of HOXA11 gene; statistical software was used to compare and analyze the differences and associations of 2D∶4D and gene polymorphisms between different genders. Results Among Ningxia Han college students, both left hand and right hand 2D∶4D were significantly higher in females than those of in males (all P<0.05), and there were no significant sex differences in right-left hand 2D∶4D; the genotypes and allele frequencies of rs7801581 locus of HOXA11 gene differed significantly between genders (all P<0.05), and none of the other locus polymorphisms showed any significant sex differences; only female left hand 2D∶4D was significantly associated with rs6461992 locus genotype in the relationship between 2D∶4D and HOXA11 polymorphisms (P<0.05). Conclusion There were significant sex differences in 2D∶4D among Han college students in Ningxia, and the rs6461992 locus polymorphism of HOXA11 gene may be associated with the formation of 2D∶4D in females.
同源盒A11基因
Homeobox A11 gene / Index finger / Ring finger / Digit ratio / Multiplex PCR / Ningxia college student
[1] Manning JT, Fink B. Sexual dimorphism in the ontogeny of second (2D) and fourth (4D) digit lengths, and digit ratio (2D∶4D)［J］. Am J Hum Biol, 2018, 30(4): 23138-23144.
［2］Fonseca C, Martelli D, Maia C, et al. Digital biomarker 2D∶4D as a predictor of cancer: A systematic review［J］. Early Hum Dev, 2022, 164(1): 105521-105532.
［3］Pasanen BE, Tomkinson JM, Dufner TJ, et al. The relationship between digit ratio (2D∶4D) and muscular fitness: A systematic review and meta-analysis［J］. Am J Hum Biol, 2022, 34(3): 23657-23669.
［4］de Sanctis V, Soliman AT, Elsedfy H, et al. Is the second to fourth digit ratio (2D∶4D) a biomarker of sex-steroids activity［J］ Pediatr Endocrinol Rev, 2017, 14(4): 378-386.
［5］Warrington NM, Shevroja E, Hemani G, et al. Genome-wide association study identifies nine novel loci for 2D∶4D finger ratio, a putative retrospective biomarker of testosterone exposure in utero［J］. Hum Mol Genet, 2018, 27(11): 2025-2038.
［6］Manning JT, Callow M, Bundred PE. Finger and toe ratios in humans and mice: implications for the aetiology of diseases influenced by HOX genes［J］. Med Hypotheses, 2003, 60(3): 340-343.
［7］Medland SE, Zayats T, Glaser B, et al. A variant in LIN28B is associated with 2D∶4D finger-length ratio, a putative retrospective biomarker of prenatal testosterone exposure［J］. Am J Hum Genet, 2010, 86(4): 519-525.
［8］Lawrance-Owen AJ, Bargary G, Bosten JM, et al. Genetic association suggests that SMOC1 mediates between prenatal sex hormones and digit ratio［J］. Hum Genet, 2013, 132(4): 415-421.
［9］Zhang J, Yang M, Luan P, et al. Associations between cytochrome P450 (CYP) gene single-nucleotide polymorphisms and second-to-fourth digit ratio in Chinese University Students［J］. Med Sci Monit, 2021, 27: 930591.
［10］Zhanbing M, Jie D, Chunyue B, et al. Association of CYP19A1 single-nucleotide polymorphism with digit ratio (2D∶4D) in a sample of men and women from Ningxia (China)［J］. Early Hum Dev, 2019, 132(4): 58-65.
［11］Manning JT, Bundred PE, Newton DJ, et al. The second to fourth digit ratio and variation in the androgen receptor gene［J］. Evolution and Human Behavior, 2003, 24(6): 399-405.
［12］Mallo M. Reassessing the role of Hox genes during vertebrate development and evolution［J］. Trends Genet, 2018, 34(3): 209-217.
［13］Xi HJ, Zhao H, Li WH, et al. Primary analysis of current studies on the digit ratio［J］. Acta Anatomica Sinica, 2012, 43 (4): 569-573. (in Chinese)
席焕久，赵红，李文慧，等. 对指长比研究现状的初步分析［J］. 解剖学报, 2012, 43(4): 569-573.
［14］Coyne SM, Manning JT, Ringer L, et al. Directional asymmetry (right-left differences) in digit ratio (2D∶4D) predict indirect aggression in women［J］. Pers Individ Differ, 2007,43(4):865-872.
［15］Pineault KM, Wellik DM. Hox genes and limb musculoskeletal development［J］. Curr Osteoporos Rep, 2014, 12(4): 420-427.
［16］Mortlock DP, Innis JW. Mutation of HOXA13 in hand-foot-genital syndrome［J］. Nat Genet, 1997, 15(2): 179-180.
［17］Small KM, Potter SS. Homeotic transformations and limb defects in Hox A11 mutant mice［J］. Genes Dev, 1993, 7(12A): 2318-2328.
［18］Kherdjemil Y, Lalonde RL, Sheth R, et al. Evolution of Hoxa11 regulation in vertebrates is linked to the pentadactyl state［J］. Nature, 2016, 539(7627): 89-92.
［19］Pearce E, Wlodarski R, Machin A, et al. Associations between neurochemical receptor genes, 2D∶4D, impulsivity and relationship quality［J］. Biol Lett, 2018, 14(12): 1-5.
［20］Zheng Z, Cohn MJ. Developmental basis of sexually dimorphic digit ratios［J］. Proc Natl Acad Sci USA, 2011, 108(39): 16289-16294.
［21］Kara M, Ozcan SS, Aran T, et al. Evaluation of endometrial receptivity by measuring HOXA-10, HOXA-11, and leukemia inhibitory factor expression in patients with polycystic ovary syndrome［J］. Gynecol Minim Invasive Ther, 2019, 8(3): 118-122.
［22］Kong C, Zhu Z, Li Y, et al. Downregulation of HOXA11 enhances endometrial cancer malignancy and cisplatin resistance via activating PTEN/AKT signaling pathway［J］. Clin Transl Oncol, 2021, 23(7): 1334-1341.
［23］Xia B, Shan M, Wang J, et al. Homeobox A11 hypermethylation indicates unfavorable prognosis in breast cancer［J］. Oncotarget, 2017,8(6):9794-9805.
［24］You L, Wu Q, Xin Z, et al. The long non-coding RNA HOXA11-AS activates ITGB3 expression to promote the migration and invasion of gastric cancer by sponging miR-124-3p［J］. Cancer Cell Int, 2021, 21(1): 576-588.
［25］Cheng Y, Xiong HY, Li YM, et al. LncRNA HOXA11-AS promotes cell growth by sponging miR-24-3p to regulate JPT1 in prostate cancer［J］. Eur Rev Med Pharmacol Sci, 2021, 25(14):4668-4677.
［26］Lu P, Wang Y, Wang F, et al. Genetic analysis of HOXA11 gene in Chinese patients with cryptorchidism［J］. Andrologia, 2018, 50(1):1-4.
［27］Deepika V, Preethy P. Evaluation of body fat composition and digit ratio (2D∶4D) in polycystic ovary syndrome in adolescents［J］. Curr Health Sci J, 2021, 47(3): 433-437.
［28］Bunevicius A. The Association of digit ratio (2D∶4D) with cancer: a systematic review and meta-analysis［J］. Dis Markers, 2018, 8(2): 7698193-7698201.
［29］Park IN, Kim TB. Impact of birth season on second-to-fourth digit ratio, prostate volume, and prostate cancer［J］. Investig Clin Urol, 2022, 63(2):192-200.
PDF(786 KB)
/
| 〈 |
|
〉 |
