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大鼠颈上神经节中Ⅰ组代谢型谷氨酸受体表达及慢性间歇性低氧对其的影响
魏茜茜1,2 李超红1,2 赵晨露1,2 唐家萍1,2 刘玉珍1,2*
Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia
目的 观察Ⅰ组代谢型谷氨酸受体（mGluR1/5）在大鼠颈上神经节（SCG）组织的表达、定位，及慢性间歇性低氧（CIH）对mGluR1/5蛋白水平的影响。 方法 12只雄性SD大鼠随机分为对照组（Ctrl）和CIH组（CIH），每组6只，模型制作6周。Western blotting检测CIH对mGluR1/5蛋白水平的影响，免疫组织化学法和免疫荧光共定位染色检测大鼠SCG中mGluR1/5的表达及分布。 结果 mGluR1/5表达于大鼠SCG，mGluR1分布于神经元和小强荧光（SIF）细胞，而在卫星胶质细胞（SGCs）、神经纤维和血管不表达；mGluR5主要分布于神经纤维，少量分布于神经元，而在SIF细胞、SGCs及血管不表达；CIH上调mGluR1/5的蛋白水平（P<0.01）。 结论 mGluR1和mGluR5均表达于大鼠SCG，但其表达部位有所不同，两者蛋白水平的升高可能参与了CIH诱导的血压升高。
Objective To observe the expression and localization of group Ⅰ metabotropic glutamate receptors (mGluR1/5) in rat superior cervical ganglion (SCG) and the effect of chronic intermittent hypoxia (CIH) on mGluR1/5 protein level. Methods Twelve male SD rats were randomly divided into control group（Ctrl）and CIH group（CIH）, 6 rats in each group. After 6 weeks of modeling, the effect of CIH on mGluR1/5 protein level was detected by Western blotting, the expression and distribution of mGluR1/5 in SCG were detected by immunohistochemistry and double-immunofluorescent staining. Results mGluR1/5 was expressed in rat SCG. mGluR1 was distributed in neurons and small intensely fluorescent (SIF) cells, but not in satellite glial cells (SGCs), nerve fibers and blood vessels, whereas mGluR5 was mainly distributed in nerve fibers and a little in neurons, but not in SGCs, SIF cells and blood vessels. CIH increased the protein levels of mGluR1/5 (P<0.01) in rat SCG. Conclusion Both mGluR1 and mGluR5 are expressed in the rat SCG, but their distribution are different, and the increased protein levels of both may be involved in CIH-induced hypertension.
Ⅰ组代谢型谷氨酸受体 /
颈上神经节 /
慢性间歇性低氧 /
高血压 /
免疫印迹法 /
大鼠
Group Ⅰ metabotropic glutamate receptor
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