体外培养大脑皮质类器官与活体大脑皮质发育的比较

Wenjuan Fan; 陈旭东; 陈永芳; 杨旭光; 金少举; 赵志军; 邓锦波

doi:10.16098/j.issn.0529-1356.2023.04.002

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解剖学报 ›› 2023, Vol. 54 ›› Issue (4) : 383-391. DOI: 10.16098/j.issn.0529-1356.2023.04.002

神经生物学

体外培养大脑皮质类器官与活体大脑皮质发育的比较

范文娟^1,2 陈旭东¹ 陈永芳¹杨旭光¹ 金少举¹ 赵志军^1* 邓锦波^1*

作者信息 +

Developmental comparison between cerebral organoids in vitro and body’s cortices in vivo

FAN Wen-juan^1,2 CHEN Xu-dong¹ CHEN Yong-fang¹ YANG Xu-guang¹ JIN Shao-ju¹ZHAO Zhi-jun^1* DENG Jin-bo^1*

Author information +

文章历史 +

摘要

目的探讨体外培养大脑类器官与体内正常大脑皮质发育的差异。方法 1. 分组：在体大脑皮质组、体外培养大脑皮质类器官组。2. 取材：收取昆明小鼠胚胎7.5 d（E7.5）、E9.5完整胚胎以及E11.5、E14.5、出生后3 d（P3）、P7大脑组织，每组标本取3只。利用小鼠诱导性多能干细胞（iPSCs）培养大脑皮质类器官，收取不同培养时间点的标本，每时间点取3个以上。3. 检测方法：通过免疫荧光染色分析各组标本不同类型细胞的分布。结果大脑类器官在组织发生、神经细胞与神经胶质细胞分化等方面与活体大脑皮质发育类似，但大脑类器官尚不具有活体大脑皮质复杂的片层结构。结论大脑类器官可以模拟在体大脑的发育，用于疾病模型建立，但大脑类器官的培养技术仍需改进。

Abstract

Objective To understand the characteristics and developmental differences between cerebral organoids in vitro and normal cerebral cortices in vivo. Methods 1. Grouping: cerebral cortices in vivo group and cultured cerebral organoids in vitro group. 2. Sample collection: cortical tissues were collected from Kunming mouse embryos at embryonic day 7.5（E7.5）, E9.5, E11.5, E14.5, and postnatal day 3 (P3) or P7. Three specimens were taken from each group. Meanwhile, cerebral organoids were cultured with mouse induced pluripotent stem cells (iPSCs), and samples at different culture time point were collected, and more than 3 samples were collected at each time point. 3. Detection method: the distribution of different types of cells in each group of specimens was analyzed by immunofluorescent staining. Results While relative similarities between in vivo cerebral cortical development and the cerebral organoids in vitro were observed, including the histogenesis, and the morphological differentiation of nerve cells and glial cells, the lamellar architecture of cerebral cortex in mouse brain was not observed in cerebral organoids. Conclusion The development of cerebral organoids in vitro has some similarity with body’s cortical development. Therefore, cerebral organoids can be used to a substitution of cortex and diseases’ models, but improvement of the existing technologies is necessary.

导出引用

范文娟陈旭东陈永芳杨旭光金少举赵志军邓锦波. 体外培养大脑皮质类器官与活体大脑皮质发育的比较[J]. 解剖学报. 2023, 54(4): 383-391 https://doi.org/10.16098/j.issn.0529-1356.2023.04.002

FAN Wen-juan CHEN Xu-dong CHEN Yong-fang YANG Xu-guang JIN Shao-ju ZHAO Zhi-jun DENG Jin-bo. Developmental comparison between cerebral organoids in vitro and body’s cortices in vivo[J]. Acta Anatomica Sinica. 2023, 54(4): 383-391 https://doi.org/10.16098/j.issn.0529-1356.2023.04.002

中图分类号： R329

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