青海省汉族妊娠期高血压疾病与维生素D受体基因FokⅠ多态性的关系

武震; 段倩; 袁明; 王雪妮; 陈文静; 李玉琴; 俄洛吉; 李建华

doi:10.16098/j.issn.0529-1356.2023.03.009

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解剖学报 ›› 2023, Vol. 54 ›› Issue (3) : 313-318. DOI: 10.16098/j.issn.0529-1356.2023.03.009

细胞和分子生物学

青海省汉族妊娠期高血压疾病与维生素D受体基因FokⅠ多态性的关系

武震^1,2段倩¹ 袁明¹ 王雪妮¹陈文静¹ 李玉琴³ 俄洛吉³ 李建华^1*

作者信息 +

Relationship between FokⅠ polymorphism of vitamin-D receptor gene and hypertensive disorder complicating pregnancy in Han nationality of Qinghai province

WU Zhen^{1, 2} DUAN Qian¹ YUAN Ming¹ WANG Xue-ni¹ CHEN Wen-jing¹ LI Yu-qin³ E-Luo-Ji³ LI Jian-hua^1*

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文章历史 +

摘要

目的探讨维生素D受体（VDR）基因单核苷酸多态性（SNP）FokⅠ（rs2228570/rs10735810）位点多态性与青海省汉族妇女妊娠期高血压疾病（HDCP）的相关性。方法选择青海省汉族HDCP患者137例（HDCP组），正常汉族孕妇146例（对照组），使用聚合酶链式反应-限制性核酸内切酶片段长度多态性（PCR-RFLP）方法检测HDCP组和对照组FokⅠ位点的基因分型并测序验证，使用SPSS 19.0统计学软件检验两组一般临床资料、基因型及等位基因频率分布差异是否具有统计学意义。结果 HDCP组和对照组FokⅠ位点FF、Ff、ff基因型频率分别为51.82%、37.96%、10.22%和34.93%、43.15%和21.92%（χ²=11.099，P<0.05），HDCP组FF基因型（OR=2.004，95% CI=1.243~3.231）频率高于对照组；HDCP组和对照组FokⅠ位点F和f等位基因频率分布差异有统计学意义（χ²=12.454，P<0.001），HDCP组F等位基因（OR=1.253，95% CI=1.105~1.421）频率高于对照组。结论 VDR基因FokⅠ位点多态性与青海省汉族HDCP相关，其中F等位基因可能是HDCP的易感基因，FF基因型可能是HDCP的易感基因型。

Abstract

Objective To investigate the relationship between single nucleotide polymorphism (SNP) FokⅠ（rs2228570/rs10735810）of vitamin D receptor (VDR) gene and hypertensive disorder complicating pregnancy (HDCP) in Han nationality women of Qinghai province. Methods A total of 137 Han nationality HDCP subjects (HDCP group) and 146 Han nationality normal pregnant subjects (control group) were selected from Qinghai province. The FokⅠ polymorphism typing in HCDP group and control group was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The mutation was confirmed by sequencing. SPSS 19.0 statistical software was used to test whether there were significant differences between two groups in general clinical data, genotype and allele frequency distribution. Results The frequency of FF Ff ff genotype of FokⅠ in HDCP group and control group were 51.82%, 37.96%, 10.22% and 34.93%, 43.15%, 21.92% respectively (χ²=11.099, P<0.05, the distribution of genotype was different significantly between the two groups), the frequency of FF genotype in HDCP group (OR=2.004, 95%CI=1.243-3.231) was higher than that in control group; The frequency distribution of alleles F and f of FokⅠ was significantly different between HDCP group and control group (χ²=12.454, P<0.001), F allele frequency in HDCP group (OR=1.253, 95%CI=1.105-1.421) was higher than that in control group. Conclusion The FokⅠ polymorphism of VDR gene is significantly correlated with hypertensive disorder complicating pregnancy of Han nationality in Qinghai. The F allele may be the susceptibility gene of HDCP, the FF genotype may be the susceptibility genotype of HDCP.

导出引用

武震段倩袁明王雪妮陈文静李玉琴俄洛吉李建华. 青海省汉族妊娠期高血压疾病与维生素D受体基因FokⅠ多态性的关系[J]. 解剖学报. 2023, 54(3): 313-318 https://doi.org/10.16098/j.issn.0529-1356.2023.03.009

WU Zhen DUAN Qian YUAN Ming WANG Xue-ni CHEN Wen-jing LI Yu-qin E-Luo-Ji LI Jian-hua.

Relationship between FokⅠ polymorphism of vitamin-D receptor gene and hypertensive disorder complicating pregnancy in Han nationality of Qinghai province

[J]. Acta Anatomica Sinica. 2023, 54(3): 313-318 https://doi.org/10.16098/j.issn.0529-1356.2023.03.009

中图分类号： R3

参考文献

［1］Yang Z, Zhang WY. Interpretation of guidelines for diagnosis and treatment of pregnancy induced hypertension (2020) ［J］. Chinese Journal of Obstetrics and Gynecology, 2020,55 (6): 425-432.(in Chinese)

杨孜,张为远.《妊娠期高血压疾病诊治指南(2020)》解读［J］.中华妇产科杂志,2020,55(6):425-432.

［2］Cheng L. Effect of different degrees of pregnancy induced hypertension syndrome on pregnancy outcome ［J］. Journal of Mathematical Medicine, 2015, 28 (10): 1445-1446. (in Chinese)

程丽.不同程度妊娠高血压综合症对妊娠结局的影响［J］.数理医药学杂志, 2015, 28(10): 1445-1446.

［3］Regitz-Zagrosek V, Roos-Hesselink JW, Bauersachs J, et al. 2018 ESC guidelines for the management of cardiovascular diseases during pregnancy: the task force for the management of cardiovascular diseases during pregnancy of the European Society of Cardiology (ESC)［J］. Eur Heart J, 2018, 39(34): 3165-3241.

［4］Lu CQ, Lin J, Yuan L, et al. Pregnancy induced hypertension and outcomes in early and moderate preterm infants［J］. Pregnancy Hypertens, 2018, 14: 68-71.

［5］Deng L, Fang BM. Clinical epidemiological analysis of hypertensive disorder complicating pregnancy［J］. Hainan Medical Journal, 2015, 26(17):2602-2603. (in Chinese)

邓莉,方碧梅.妊娠期高血压疾病的临床流行病学分析［J］. 海南医学, 2015, 26(17):2602-2603.

［6］Pu J. Changes in the composition of maternal death causes in China ［J］. Journal of Practical Obstetrics and Gynecology, 2021,37 (3): 161-165. (in Chinese)

蒲杰.我国孕产妇死因构成的变迁［J］.实用妇产科杂志,2021,37(3):161-165.

［7］Awamleh Z, Han VKM. Identification of miR-210-5p in human placentae from pregnancies complicated by preeclampsia and intrauterine growth restriction, and its potential role in the pregnancy complications［J］. Pregnancy Hypertens, 2020, 19: 159-168.

［8］Liu J, Li W, Wang YQ. Bibliometric analysis of international research hotspots of pregnancy induced hypertension ［J］. China Maternal and Child Health Research, 2017,28 (3): 262-266. (in Chinese)

刘珏,李维,王永清.妊娠期高血压疾病国际研究热点的文献计量分析［J］.中国妇幼健康研究,2017,28(3):262-266.

［9］El-Shal AS, Shalaby SM, Aly NM, et al. Genetic variation in the vitamin D receptor gene and vitamin D serum levels in Egyptian women with polycystic ovary syndrome［J］. Mol Biol Rep, 2013, 40(11): 6063-6073.

［10］Haddad S. Vitamin-D receptor (VDR) gene polymorphism (FokI) in Syrian healthy population［J］. Gene Reports, 2021, 23: 101083.

［11］Li XW, Li J, Zhu QY. Correlation between serum vitamin D level and vitamin D receptor gene polymorphism in pregnant women with preeclampsia ［J］. Journal of Medical Research, 2021,50 (1): 104-108. (in Chinese)

李祥雯,李晶,朱启英.子痫前期孕妇血清维生素D水平与维生素D受体基因多态性的相关性［J］.医学研究杂志,2021,50(1):104-108.

［12］Robinson CJ, Alanis MC, Wagner CL, et al. Plasma 25-hydroxyvitamin D levels in early-onset severe preeclampsia［J］. Am J Obstet Gynecol, 2010, 203(4): 366. e1-6.

［13］Zhou WZh, Qu HM. Role of bioactive factors in the pathogenesis of pregnancy induced hypertension ［J］. International Journal of Obstetrics and Gynecology, 2021,48 (4): 361-366. (in Chinese)

周文哲,曲洪美.生物活性因子在妊娠期高血压疾病发病机制中的作用［J］.国际妇产科学杂志,2021,48(4):361-366.

［14］Shin JS, Choi MY, Longtine MS, et al. Vitamin D effects on pregnancy and the placenta［J］. Placenta, 2010, 31(12): 1027-1034.

［15］Cim N, Kurdoglu M, Ege S, et al. An analysis on the roles of angiogenesis-related factors including serum vitamin D, soluble endoglin (sEng), soluble fms-like tyrosine kinase 1 (sFlt1), and vascular endothelial growth factor (VEGF) in the diagnosis and severity of late-onset preeclampsia［J］. J Matern Fetal Neonatal Med, 2017, 30(13): 1602-1607.

［16］Wang Y, Liu RX, Liu H. Association of adiponectin gene polymorphisms with hypertensive disorder complicating pregnancy and disorders of lipid metabolism［J］. Genet Mol Res, 2015, 14(4): 15213-15223.

［17］Elhoseiny SM, Morgan DS, Rabie AM, et al. Vitamin D receptor (VDR) gene polymorphisms (FokI, BsmI) and their relation to vitamin D status in pediatrics βeta thalassemia major［J］. Indian J Hematol Blood Transfus, 2016, 32(2): 228-238.

［18］McGrath JJ, Saha S, Burne THJ, et al. A systematic review of the association between common single nucleotide polymorphisms and 25-hydroxyvitamin D concentrations［J］. J Steroid Biochem Mol Biol, 2010, 121(1-2): 471-477.

［19］Purswani JM, Gala P, Dwarkanath P, et al. The role of vitamin D in pre-eclampsia: a systematic review［J］. BMC Pregnancy Childbirth, 2017, 17(1): 1-15.

［20］O’Callaghan KM, Kiely M. Systematic review of vitamin D and hypertensive disorders of pregnancy［J］. Nutrients, 2018, 10(3): 294.

［21］Rezavand N, Tabarok S, Rahimi Z, et al. The effect of VDR gene polymorphisms and vitamin D level on blood pressure, risk of preeclampsia, gestational age, and body mass index［J］. J Cell Biochem, 2019, 120(4): 6441-6448.

［22］Rezende VB, Sandrim VC, Palei AC, et al. Vitamin D receptor polymorphisms in hypertensive disorders of pregnancy［J］. Mol Biol Rep, 2012, 39(12): 10903-10906.